体外雄性曼氏血吸虫对来自雌性血吸虫提取物的定向。

In vitro orientation of maleSchistosoma mansoni to extracts derived from female schistosomes.

机构信息

Department of Immunology and Infectious Diseases, The Johns Hopkins University, 21205, Baltimore, Maryland.

出版信息

J Chem Ecol. 1986 Aug;12(8):1729-38. doi: 10.1007/BF01022378.

Abstract

Chemical orientation of adult maleSchistosoma mansoni was studied during cultivation in vitro. Directional preference was assessed in culture vessels marked with compass coordinates by the application of circular statistics for determining clustering and orientation to a predicted direction. Organic solvent extracts of fresh female schistosomes and supernatant fluids from 72-hr cultures of female parasites were tested for potential chemotactic activity. Analysis showed significant (P < 0.05) directional preference and clustering of male worms towards test compounds at all time periods (24, 48, and 72 hr) with one mixture of female extracts and at one of three time periods with a second female extract. Male worms did not respond to ecdysone, cholesterol, or solvent controls by orienting in predicted direction, although clustering was observed on two of 12 occasions. These results indicate the presence of a chemoattractant compound(s) in female extracts.

摘要

在体外培养过程中研究了成年雄性曼氏血吸虫的化学定向。通过应用圆形统计来确定聚类和朝向预测方向的能力，在标记有罗盘坐标的培养容器中评估定向偏好。对新鲜雌性血吸虫的有机溶剂提取物和 72 小时雌性寄生虫培养物的上清液进行了潜在趋化活性测试。分析显示，在所有时间段（24、48 和 72 小时），雄性蠕虫对测试化合物表现出明显的（P<0.05）定向偏好和聚类，其中一种雌性提取物混合物和三种雌性提取物中的一种在一个时间段内表现出这种情况。尽管在 12 次中有两次观察到聚类，但雄性蠕虫不会通过朝向预测方向对蜕皮激素、胆固醇或溶剂对照做出反应。这些结果表明雌性提取物中存在一种趋化剂化合物（或化合物混合物）。