Smith P A, Thompson E L, Zidichouski J A
Neurosci Lett. 1986 Oct 30;71(1):72-6. doi: 10.1016/0304-3940(86)90259-4.
The potassium-activated hyperpolarization (KH) was used as an index of electrogenic Na+ pumping in bullfrog sympathetic ganglia. This response was evoked by storing ganglia in K-free Ringer's solution and briefly introducing normal Ringer's solution containing 2 mM K+ at regular intervals. The apparent EC50 for K+ was 2.21 mM (range 0.88-3.54 mM, for n = 5) and at least 10 mM K+ was required to produce a maximal KH response. Adrenaline, which produces membrane hyperpolarization by increasing K+ conductance (gK), increased the amplitude of KH responses. When the K+ efflux accompanying the adrenaline-induced hyperpolarization (AdH) was blocked with 2 mM Ba2+, the KH was no longer potentiated. It is suggested that the K+ moving out of the cells during the AdH accumulates extracellularly and stimulates the Na+ pump.
钾激活超极化（KH）被用作牛蛙交感神经节中电生性钠泵的指标。通过将神经节储存在无钾林格氏液中，并定期短暂引入含2 mM钾的正常林格氏液来诱发这种反应。钾的表观半数有效浓度（EC50）为2.21 mM（n = 5时范围为0.88 - 3.54 mM），至少需要10 mM钾才能产生最大的KH反应。肾上腺素通过增加钾电导（gK）产生膜超极化，增加了KH反应的幅度。当用2 mM Ba2+阻断伴随肾上腺素诱导超极化（AdH）的钾外流时，KH不再增强。提示在AdH期间从细胞中移出的钾在细胞外积累并刺激钠泵。
