Locsey L, Seres I, Sztanek F, Harangi M, Padra J, Kovacs D, Fedor R, Asztalos L, Paragh G
Institute of Surgery, University of Debrecen Medical and Health Science Center, Debrecen, Hungary.
Transplant Proc. 2013;45(10):3685-7. doi: 10.1016/j.transproceed.2013.10.003.
Paraoxonase lactonase activity protects against homocysteinylation; therefore, it can be a potential contributing factor to prevent atherosclerosis. We aimed to determine paraoxonase and HTLase activities and to clarify the relationship between HTLase activity and some cardiovascular risk factors, such as homocysteine, cystatin C asymmetric dimethylarginine (ADMA), and adipokines both in hemo dialyzed and transplanted patients. Among 114 hemodialyzed, 80 transplanted and 64 healthy control subjects, we investigated body mass index (BMI) as well as fasting serum contents of urea, uric acid, creatinine, cystatin C, homocysteine, glucose, lipids, total protein and albumin. Serum paraoxonase (PON 1) and HTLase activities were measured spectrophotometrically. ADMA, ADPN adiponectin, leptin (LEP) levels was determined with a sandwich enzyme-linked immunosorbent assay method. Dyslipidemic patients showed hypercholesterolemia, and high low-density lipoprotein (LDL); parallel with improved renal function, they displayed decreased cystatin C and homocysteine levels (P < .001). There was a significant negative correlation between PON 1 activity and cystatin C and homocysteine concentrations (P < .05). Obese patients revealed significantly higher LDL (P < .05) and leptin concentrations (P < .01). There was a significant positive correlation between PON 1 activity and adiponectin levels (P = .0276). Both dialyzed and transplanted patients displayed significantly lower HTLase activities compared to the control group (P < .001), particularly lower HTLase and PON 1 activities in dialyzed subjects compared with the transplanted group (P < .05). HTLase activity showed significant negative correlations with ADMA levels among the whole study population (P < .001), whereas positive associations were noted between PON 1 and HTLase activities (P < .001). HTLase activity may be a new predictor of cardiovascular risk in renal failure although it is modulated by other risk factors.
对氧磷酶内酯酶活性可预防同型半胱氨酸化；因此，它可能是预防动脉粥样硬化的一个潜在因素。我们旨在测定对氧磷酶和HTLase活性，并阐明HTLase活性与一些心血管危险因素之间的关系，如同型半胱氨酸、胱抑素C、不对称二甲基精氨酸（ADMA）以及血液透析患者和移植患者体内的脂肪因子。在114名血液透析患者、80名移植患者和64名健康对照者中，我们调查了体重指数（BMI）以及空腹血清中尿素、尿酸、肌酐、胱抑素C、同型半胱氨酸、葡萄糖、脂质、总蛋白和白蛋白的含量。采用分光光度法测定血清对氧磷酶（PON 1）和HTLase活性。采用夹心酶联免疫吸附测定法测定ADMA、脂联素（ADPN）、瘦素（LEP）水平。血脂异常患者表现为高胆固醇血症和高低密度脂蛋白（LDL）；随着肾功能的改善，他们的胱抑素C和同型半胱氨酸水平降低（P < 0.001）。PON 1活性与胱抑素C和同型半胱氨酸浓度之间存在显著负相关（P < 0.05）。肥胖患者的LDL（P < 0.05）和瘦素浓度显著更高（P < 0.01）。PON 1活性与脂联素水平之间存在显著正相关（P = 0.0276）。与对照组相比，血液透析患者和移植患者的HTLase活性均显著降低（P < 0.001），与移植组相比，血液透析患者的HTLase和PON 1活性尤其降低（P < 0.05）。在整个研究人群中，HTLase活性与ADMA水平呈显著负相关（P < 0.001），而PON 1与HTLase活性之间呈正相关（P < 0.001）。尽管HTLase活性受其他危险因素的调节，但它可能是肾衰竭心血管风险的一个新预测指标。
