[增强绿色荧光蛋白报告基因标记的辛德毕斯病毒的构建与鉴定]
[Construction and characterization of EGFP reporter gene labeled Sindbis virus].
作者信息
Deng Ling-Ling, Li Jiang-Jiao, Wei Yan, Wang Huan-Qin, Zhang Feng-Juan, Sun Ji-Guo, Chen Chang, Zhu Wu-Yang, Liang Guo-Dong
机构信息
College of Life Science & Technology, Beijing University of Chemical Technology, Beijing 100029, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Jun;27(3):228-30.
OBJECTIVE
To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).
METHODS
The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.
RESULTS
We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.
CONCLUSION
The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.
目的
构建并鉴定增强型绿色荧光蛋白（EGFP）报告基因标记的辛德毕斯病毒（SINV）。
方法
采用多融合长片段PCR方法将报告基因EGFP插入感染性克隆pBR-XJ160的基因组中。然后应用反向遗传操作技术拯救并获得EGFP标记的SINV。
结果
我们成功获得了标记的SINV，其具有良好的荧光表达特性和遗传稳定性。
结论
标记病毒可在活细胞和活体中观察到，这为研究病毒的细胞和组织嗜性及生物学功能提供了良好工具。本研究为进一步研究SINV的细胞嗜性、生物学功能及感染机制奠定了基础。
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