Lin-ve CD34 和 CD117 细胞群体的特征表明骨髓来源的干细胞中表达增加。
Characterization of Lin-ve CD34 and CD117 cell population reveals an increased expression in bone marrow derived stem cells.
机构信息
Neuroscience Research Lab, Department of Neurology, PGIMER, Chandigarh- 160012, India.
出版信息
Curr Neurovasc Res. 2014 Feb;11(1):68-74. doi: 10.2174/1567202610666131209110035.
The purpose of the study was to evaluate the expression of CD45, CD34, Sca-1 and CD117 in mouse bone marrow, Lin-ve and Lin+ve population. Bone marrow cells were isolated from C57/BL6J mouse and mononuclear population was separated from rest of the cell population. With the help of Magnetic associated cell sorter (MACS), Linve and Lin+ve cells were separated from the bone marrow. The expression of CD45, CD34, Sca1 and CD117 was evaluated in bone marrow, Lin-ve and Lin+ve population by flow cytometry. We found a significant increase in the expression of CD34 and CD117 in Lin-ve as compared to the bone marrow and Lin+ve population. These findings suggest that Lin-ve population has higher expression of stem cell progenitor markers and could be useful for tissue repair and regeneration.
本研究旨在评估 CD45、CD34、Sca-1 和 CD117 在小鼠骨髓、Lin-ve 和 Lin+ve 群体中的表达。从 C57/BL6J 小鼠中分离骨髓细胞，并从其余细胞群体中分离出单核细胞群体。借助磁珠细胞分选（MACS），从骨髓中分离出 Lin-ve 和 Lin+ve 细胞。通过流式细胞术评估骨髓、Lin-ve 和 Lin+ve 群体中 CD45、CD34、Sca1 和 CD117 的表达。我们发现与骨髓和 Lin+ve 群体相比，Lin-ve 中 CD34 和 CD117 的表达显著增加。这些发现表明 Lin-ve 群体具有更高的干细胞祖细胞标志物表达水平，可能对组织修复和再生有用。
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