[口咽鳞状细胞癌中HPV-DNA状态与p16蛋白表达的关系及其临床意义]

[Relationship between HPV-DNA status and p16 protein expression in oropharyngeal squamous cell carcinoma and their clinical significance].

作者信息

Huang Hui, Zhang Bin, Chen Wen, Zou Shuang-mei, Xu Zhen-gang

机构信息

Department of Head and Neck Surgery, Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

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出版信息

Zhonghua Zhong Liu Za Zhi. 2013 Sep;35(9):684-8.

PMID:24332056

Abstract

OBJECTIVE

To investigate the relationship between HPV-DNA status and p16 protein expression in oropharyngeal squamous cell carcinoma (OSCC) and their clinical significance.

METHODS

Sixty-six patients with oropharyngeal squamous cell carcinomas treated in the Cancer Hospital of Chinese Academy of Medical Sciences from Jan. 1999 to Dec. 2009 were included in this study. Their formalin-fixed and paraffin-embedded tumor tissue blocks met the eligibility criteria and were used in this study. A "sandwich" technique was used to prepare paraffin sections for HPV-DNA analysis. HPV-DNA was detected using the SPF10 LiPA25 version 1 assay. The expression of p16 protein was detected by immunohistochemistry. The survival rates of patients with different HPV-DNA and p16 protein status were analyzed.

RESULTS

HPV-DNA was detected in 11 (16.7%) of all specimens. Expression of p16 protein was detected in 9 of the 11 patients with HPV-positive tumors, and in 12 patients of 55 HPV-negative tumors. The expression of p16 protein was highly correlated with the presence of HPV-DNA (P < 0.001). The tumors were classified into three groups based on the p16 protein expression and HPV-DNA status: group A (9 patients): HPV(+) and p16 protein(+); group B (14 patients): HPV-DNA(+)/p16 protein(-) or HPV-DNA(-)/p16 protein(+); and group C (43 patients): HPV-DNA(-)/p16 protein(-). The 3-year OS rates of these 3 groups were 100%, 77.8% and 42.0% (P = 0.001), and their DSS rates were 100%, 77.8% and 46.4%, respectively(P = 0.004).

CONCLUSIONS

In oropharyngeal squamous cell carcinomas, p16 protein expression is highly correlated with the presence of HPV-DNA, and might be a surrogate marker for HPV-positive OSCC. Combination of p16 protein and HPV-DNA status detection may help to more accurately stratify oropharyngeal carcinomas and predict their prognosis.

摘要

目的

探讨口咽鳞状细胞癌（OSCC）中HPV-DNA状态与p16蛋白表达之间的关系及其临床意义。

方法

本研究纳入了1999年1月至2009年12月在中国医学科学院肿瘤医院接受治疗的66例口咽鳞状细胞癌患者。他们的福尔马林固定石蜡包埋肿瘤组织块符合纳入标准并用于本研究。采用“夹心”技术制备用于HPV-DNA分析的石蜡切片。使用SPF10 LiPA25版本1检测法检测HPV-DNA。通过免疫组织化学检测p16蛋白的表达。分析不同HPV-DNA和p16蛋白状态患者的生存率。

结果

在所有标本中，11例（16.7%）检测到HPV-DNA。在11例HPV阳性肿瘤患者中的9例以及55例HPV阴性肿瘤患者中的12例检测到p16蛋白表达。p16蛋白表达与HPV-DNA的存在高度相关（P < 0.001）。根据p16蛋白表达和HPV-DNA状态将肿瘤分为三组：A组（9例患者）：HPV(+)且p16蛋白(+)；B组（14例患者）：HPV-DNA(+)/p16蛋白(-)或HPV-DNA(-)/p16蛋白(+)；C组（43例患者）：HPV-DNA(-)/p16蛋白(-)。这三组的3年总生存率分别为100%、77.8%和42.0%（P = 0.001），其疾病特异性生存率分别为100%、77.8%和46.4%（P = 0.004）。