Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan.
Mar Drugs. 2013 Dec 11;11(12):5024-35. doi: 10.3390/md11125024.
Chondroitin sulfate (CS) has been suggested to be involved in bone formation and mineralization processes. A previous study showed that squid-derived CS (sqCS) has osteoblastogenesis ability in cooperation with bone morphogenetic protein (BMP)-4 in vitro. However, in vivo, osteogenic potential has not been verified. In this study, we created a critical-sized bone defect in the rat calvaria and implanted sqCS-loaded gelatin hydrogel sponges (Gel) into the defect with or without BMP-4 (CS/BMP/Gel and CS/Gel, respectively). At 15 weeks, bone repair rate of CS/Gel-treated defects and CS/BMP/Gel-treated defects were 47.2% and 51.1%, respectively, whereas empty defects and defects with untreated sponges showed significantly less bone ingrowth. The intensity of von Kossa staining of the regenerated bone was less than that of the original one. Mineral apposition rates at 9 to 10 weeks were not significantly different between all treatment groups. Although bone repair was not completed, sqCS stimulated bone regeneration without BMP-4 and without external mesenchymal cells or preosteoblasts. Therefore, sqCS is a promising substance for promotion of osteogenesis.
硫酸软骨素（CS）被认为参与骨形成和矿化过程。先前的一项研究表明，鱿鱼来源的 CS（sqCS）在体外与骨形态发生蛋白（BMP）-4 合作具有成骨能力。然而，在体内，其成骨潜力尚未得到验证。在这项研究中，我们在大鼠颅骨中创建了一个临界大小的骨缺损，并将负载 sqCS 的明胶水凝胶海绵（Gel）植入缺损中，有无 BMP-4（CS/BMP/Gel 和 CS/Gel）。在 15 周时，CS/Gel 处理的缺损和 CS/BMP/Gel 处理的缺损的骨修复率分别为 47.2%和 51.1%，而空缺损和未处理海绵的缺损的骨内生长明显较少。再生骨的 von Kossa 染色强度低于原始骨。在 9 至 10 周时，所有治疗组的矿化速率没有显著差异。尽管骨修复没有完成，但 sqCS 在没有 BMP-4 和没有外间质细胞或成骨前体细胞的情况下刺激骨再生。因此，sqCS 是一种有前途的促进成骨物质。
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