Kudo Y, Yanagawa R, Hiramune T
Vet Microbiol. 1987 Jan;13(1):75-85. doi: 10.1016/0378-1135(87)90101-5.
Five monoclonal antibodies against pili of Corynebacterium renale 115 P+ (piliated clone) and two monoclonal antibodies against pili of C. pilosum 92 P+ (piliated clone) were produced. These antibodies bound to pili of the homologous strain in in enzyme-linked immunosorbent assay (ELISA) and agglutinated P+ but not P- (non-piliated clone) of each homologous strain. The five monoclonal antibodies against C. renale 115 P+ pili were divided into 2 groups, comprising 16/5, 160/1 and 32/6 and 13/4 and B20/3, based on the results of a competitive binding assay. The results may indicate the presence of at least 2 distinct antigenic areas on the pilus of C. renale 115 P+. The monoclonal antibodies of the first group inhibited adhesion of C. renale 115 P+ bacteria to the epithelial cells of bovine vulva, while the second group did not. Two monoclonal antibodies against C. pilosum 92 P+ pili recognized the same area on the pilus of C. pilosum 92 P+, and inhibited the adhesion of C. pilosum 92 P+ bacteria to the epithelial cells of bovine vulva. The adhesion of these bacteria was inhibited by the monoclonal antibodies in the form of IgG as well as by the Fab fragment. The strains of C. renale and C. pilosum which reacted with each of the anti-C. renale 115 P+ pili and anti-C. pilosum 92 P+ pili monoclonal antibodies were small in number and of restricted distribution.
制备了5种针对肾棒状杆菌115 P+（有菌毛克隆）菌毛的单克隆抗体和2种针对毛样棒状杆菌92 P+（有菌毛克隆）菌毛的单克隆抗体。在酶联免疫吸附测定（ELISA）中，这些抗体与同源菌株的菌毛结合，并凝集每种同源菌株的P+而非P-（无菌毛克隆）。根据竞争结合试验结果，针对肾棒状杆菌115 P+菌毛的5种单克隆抗体分为2组，包括16/5、160/1和32/6以及13/4和B20/3。结果可能表明肾棒状杆菌115 P+菌毛上至少存在2个不同的抗原区域。第一组单克隆抗体抑制肾棒状杆菌115 P+细菌与牛外阴上皮细胞的黏附，而第二组则无此作用。2种针对毛样棒状杆菌92 P+菌毛的单克隆抗体识别毛样棒状杆菌92 P+菌毛上的同一区域，并抑制毛样棒状杆菌92 P+细菌与牛外阴上皮细胞的黏附。这些细菌的黏附受到IgG形式的单克隆抗体以及Fab片段的抑制。与每种抗肾棒状杆菌115 P+菌毛和抗毛样棒状杆菌92 P+菌毛单克隆抗体发生反应的肾棒状杆菌和毛样棒状杆菌菌株数量较少且分布有限。
