Department of Neurology, Second Affiliated Hospital of Soochow University, No. 1055, San Xiang Road, Suzhou, 215004, Jiangsu, China.
Cell Biochem Biophys. 2014 Jun;69(2):333-40. doi: 10.1007/s12013-013-9804-4.
One of the crucial challenges in medicine is the treatment and rehabilitation of spinal cord injury (SCI). In this study, we established a stable and reproducible acute spinal cord injury model in adult rats. The SCI was inflicted by our self-innovated spinal cord impact device controlled by electrical circuit. The Basso, Beattie, and Bresnahan Locomotor Rating Scale (BBB) score, electrophysiology, histological, and immunohistochemical changes after SCI were observed. The BBB score of the injured rats began to increase from the 3rd day of SCI and reached at the score 7.2 ± 1.3 at the 28th day. The latency of cortical somatosensory evoked potentials (CSEP) was not observed 2 and 6 h after injury, but appeared 24 h after injury which was significantly prolonged. It recovered from day 3 gradually to 27.3 ± 2.7 ms on day 28. H&E staining showed that the structure of gray and white matter was disrupted after the SCI. The result also showed dramatic neuron degenerations, cellular swelling, and the proliferation of glial cells. The immunohistochemical analysis showed that the expression of neuron specific enolase (NSE) and neurofilament 200 (NF200) started lowering at 2 h and dropped to the bottom at 24 h. Their expression rebound from day 3 and yet to the original level at day 28 (P < 0.05). The number of cells expressing glial fibrillary acidic protein (GFAP) hiked from day 3, peaked at day 14, and began recovering from day 28 (P < 0.05). The changes of NSE, NF200, GFAP, and CSEP were significantly associated with the BBB score (P < 0.05). In conclusion, our self-innovated device can reproduce the injury model stably. The changes of NSE, NF, and GFAP after spinal cord injury reflect the characteristics of pathological change, which are closely associated with the functional recovery from the spinal cord injury.
医学面临的一个关键挑战是脊髓损伤（SCI）的治疗和康复。在本研究中，我们建立了一种稳定且可重复的成年大鼠急性脊髓损伤模型。SCI 是由我们自行创新的脊髓撞击装置通过电路控制造成的。观察 SCI 后大鼠的 Basso、Beattie 和 Bresnahan 运动评分（BBB）、电生理学、组织学和免疫组织化学变化。受伤大鼠的 BBB 评分从 SCI 后第 3 天开始增加，到第 28 天达到 7.2±1.3 分。皮质体感诱发电位（CSEP）的潜伏期在损伤后 2 和 6 小时未观察到，但在损伤后 24 小时出现，潜伏期明显延长。它从第 3 天开始逐渐恢复，到第 28 天恢复到 27.3±2.7 ms。H&E 染色显示 SCI 后灰质和白质的结构被破坏。结果还显示神经元明显退化、细胞肿胀和胶质细胞增殖。免疫组织化学分析显示神经元特异性烯醇化酶（NSE）和神经丝 200（NF200）的表达在 2 小时开始下降，24 小时降至最低点。它们的表达从第 3 天开始反弹，但在第 28 天仍未恢复到原来的水平（P<0.05）。胶质纤维酸性蛋白（GFAP）表达的细胞数量从第 3 天开始增加，在第 14 天达到峰值，从第 28 天开始恢复（P<0.05）。NSE、NF、GFAP 和 CSEP 的变化与 BBB 评分显著相关（P<0.05）。总之，我们自行创新的装置可以稳定地复制损伤模型。脊髓损伤后 NSE、NF 和 GFAP 的变化反映了病理变化的特征，与脊髓损伤后的功能恢复密切相关。
