Miron Nicolae, Feldrihan Vasile, Berindan-Neagoe Ioana, Cristea Victor
Department of Immunology, University of Medicine and Pharmacy "Iuliu Haţieganu" , Cluj-Napoca , Romania .
Immunol Invest. 2014;43(3):267-77. doi: 10.3109/08820139.2013.868474. Epub 2013 Dec 19.
Oral tolerance is the biological process explaining the non-responsiveness of gut lymphoid tissue to intestinal content. Our study tested a new approach for the enhancement of oral tolerance to a multiple sclerosis-triggering auto-antigen-myelin basic protein, by its oral administration with the Staphylococcal enterotoxin A.
Immune tolerance thus stimulated was assessed in adult BALB/c mice, by measuring different cytokines from the supernatant of mesenteric lymph nodes cells (IFN-γ, IL-4, IL-10, IL-17, and TGF-β), and in a SJL/E mouse model of experimental autoimmune encephalomyelitis, by evaluating the development of regulatory T cells in mesenteric lymph nodes and the clinical outcome of the intervention.
We obtained a significant rise in the levels of IL-10 and TGF-β compared with control and a significant decrease of IFN-γ, IL-4 (p < 0.05). Regulatory T cells were increased compared with control (p < 0.05). These results were attributable both to myelin basic protein and to Staphylococcal enterotoxin A. The clinical outcome of experimental autoimmune encephalomyelitis was influenced only by the administration of myelin basic protein.
In our experiment, Staphylococcal enterotoxin A enhanced the immune tolerance to myelin basic protein in the gut mucosa, but had no impact on the clinical evolution of experimental autoimmune encephalomyelitis.
口服耐受是一种生物学过程，可解释肠道淋巴组织对肠内容物无反应的现象。我们的研究测试了一种新方法，即通过将葡萄球菌肠毒素A与引发多发性硬化症的自身抗原——髓鞘碱性蛋白一起口服，来增强对其的口服耐受。
通过测量肠系膜淋巴结细胞上清液中的不同细胞因子（IFN-γ、IL-4、IL-10、IL-17和TGF-β），在成年BALB/c小鼠中评估由此刺激产生的免疫耐受；在实验性自身免疫性脑脊髓炎的SJL/E小鼠模型中，通过评估肠系膜淋巴结中调节性T细胞的发育情况以及干预的临床结果来进行评估。
与对照组相比，我们发现IL-10和TGF-β水平显著升高，IFN-γ、IL-4水平显著降低（p < 0.05）。与对照组相比，调节性T细胞增加（p < 0.05）。这些结果既归因于髓鞘碱性蛋白，也归因于葡萄球菌肠毒素A。实验性自身免疫性脑脊髓炎的临床结果仅受髓鞘碱性蛋白给药的影响。
在我们的实验中，葡萄球菌肠毒素A增强了肠道黏膜对髓鞘碱性蛋白的免疫耐受，但对实验性自身免疫性脑脊髓炎的临床进展没有影响。
