Pawar Deepali D, Mehta Dhoom S
Department of Periodontics, Bapuji Dental College and Hospital, Davangere, India.
J Investig Clin Dent. 2015 May;6(2):118-24. doi: 10.1111/jicd.12072. Epub 2013 Dec 20.
The aim of the present study was to estimate the gingival crevicular fluid (GCF) levels of matrix metalloproteinases (MMP)-3 and -13 in periodontally-healthy controls and chronic periodontitis (CP) patients, and also to investigate the effect of phase 1 periodontal therapy on MMP-3 and -13 levels in CP patients.
Fifty-five systemically-healthy patients were divided into two groups: group 1 (healthy) and group 2 (CP). The recording of clinical parameters and GCF sampling was done at baseline for both groups and again at 6 weeks post-therapy for group 2. The MMP level was determined by ELISA.
A significant increase in the mean MMP-3 and -13 was found between healthy and CP patients. There was a statistically-significant reduction of GCF MMP-3 and -13 concentration after periodontal therapy in the CP group. A positive correlation was found between clinical parameters and GCF MMP-3 and -13 levels.
A lower concentration of GCF MMP-3 and -13 was found in healthy patients, and a higher concentration was noted for CP patients, which was reduced after periodontal therapy. This indicates the important role played by these MMP in periodontal destruction. Thus, MMP-3 and -13 could be used as inflammatory biomarkers in diagnosing periodontal disease severity.
本研究旨在评估牙周健康对照者和慢性牙周炎（CP）患者龈沟液（GCF）中基质金属蛋白酶（MMP）-3和-13的水平，并研究一期牙周治疗对CP患者MMP-3和-13水平的影响。
55例全身健康的患者被分为两组：第1组（健康组）和第2组（CP组）。两组均在基线时记录临床参数并采集GCF样本，第2组在治疗后6周再次记录和采样。采用酶联免疫吸附测定法（ELISA）测定MMP水平。
健康组和CP组患者的MMP-3和-13平均水平有显著升高。CP组牙周治疗后，GCF中MMP-3和-13的浓度有统计学意义的降低。临床参数与GCF中MMP-3和-13水平之间呈正相关。
健康患者GCF中MMP-3和-13的浓度较低，CP患者浓度较高，牙周治疗后浓度降低。这表明这些MMP在牙周组织破坏中起重要作用。因此，MMP-3和-13可作为诊断牙周疾病严重程度的炎症生物标志物。
