人促黄体生成素α亚基和β亚基放射免疫测定的特点

Characteristics of radioimmunoassays for the alpha- and beta-subunits of human luteinizing hormone.

作者信息

St-Arnaud R, Lachance R, Labrie F

出版信息

J Immunoassay. 1987;8(1):97-114. doi: 10.1080/01971528708063056.

DOI:10.1080/01971528708063056

Abstract

The binding characteristics and specificities of the National Hormone and Pituitary Program (NHPP) kits for the radioimmunoassay of the alpha- and beta-subunits of human luteinizing hormone (hLH-alpha and hLH-beta) were studied, as well as the specificities of the anti-hLH and anti-human follicle stimulating hormone (anti-hFSH) antisera distributed by the same organization. The affinity constants of the anti-hLH-alpha and anti-hLH-beta antisera were calculated at 157 +/- 8.4 nM-1 and 109 +/- 7.4 nM-1, respectively. Both antisera were highly specific with regard to the other subunit. However, in the homologous hLH-alpha RIA, native hLH cross-reacted at 21.9%, hFSH at 17.5% and hTSH at 7.9%. The alpha-subunit of the human chorionic gonadotropin, hCG-alpha, was equipotent with the hLH-alpha standard in this assay. In the homologous hLH-beta RIA, hLH showed a cross-reactivity of 14.7% while the cross-reactivities of hCG-beta, hFSH and hTSH were 3.5%, 1.2% and 0.6%, respectively. The anti-hFSH antiserum was highly specific, while the anti-hLH antiserum showed non parallel competition curves. With this knowledge of the specificity of each antiserum, corrections can be properly made for the assays of hLH, hLH-alpha and hLH-beta while the hFSH RIA can be used without correction for the presence of the three other components.

摘要

研究了美国国立激素与垂体项目（NHPP）试剂盒用于人促黄体生成素α亚基和β亚基（hLH-α和hLH-β）放射免疫测定的结合特性和特异性，以及同一机构分发的抗hLH和抗人促卵泡生成素（抗hFSH）抗血清的特异性。抗hLH-α和抗hLH-β抗血清的亲和常数分别计算为157±8.4nM⁻¹和109±7.4nM⁻¹。两种抗血清对另一个亚基都具有高度特异性。然而，在同源的hLH-α放射免疫分析中，天然hLH的交叉反应率为21.9%，hFSH为17.5%，hTSH为7.9%。人绒毛膜促性腺激素的α亚基hCG-α在该分析中与hLH-α标准品等效。在同源的hLH-β放射免疫分析中，hLH的交叉反应率为14.7%，而hCG-β、hFSH和hTSH的交叉反应率分别为3.5%、1.2%和0.6%。抗hFSH抗血清具有高度特异性，而抗hLH抗血清显示出非平行竞争曲线。基于每种抗血清特异性的这些知识，在hLH、hLH-α和hLH-β的测定中可以进行适当校正，而hFSH放射免疫分析在存在其他三种成分时无需校正即可使用。