Iida K, Mitomo K, Fujita T, Tamura N
J Immunol Methods. 1987 Apr 2;98(1):23-8. doi: 10.1016/0022-1759(87)90431-5.
A solid-phase anti-C3 assay detecting immune complexes associated with C3 fragments is described. Two monoclonal antibodies against C3 fragments are used; one recognizes an epitope expressed on C3b, C3c and C3i, the other recognizes an epitope expressed on C3dg and iC3b. Combining these monoclonal anti-C3s with antibodies against class-specific immunoglobulins in enzyme-linked immunosorbent assay (ELISA), immune complexes (ICs) are detected in six distinguished forms; C3b-IgG-IC, iC3b/C3dg-IgG-IC, C3b-IgA-IC, iC3b/C3dg-IgA-IC, C3b-IgM-IC and iC3b/C3dg-IgM-IC. About three-fourths of the plasma samples from patients with active SLE or IgA-nephritis were found positive in one or more types of ICs. IgA-type ICs were found very frequently in patients with autoimmune or renal diseases.
本文描述了一种检测与C3片段相关的免疫复合物的固相抗C3检测法。使用了两种针对C3片段的单克隆抗体；一种识别在C3b、C3c和C3i上表达的表位，另一种识别在C3dg和iC3b上表达的表位。在酶联免疫吸附测定（ELISA）中将这些抗C3单克隆抗体与针对类特异性免疫球蛋白的抗体相结合，可检测到六种不同形式的免疫复合物（ICs）；C3b-IgG-IC、iC3b/C3dg-IgG-IC、C3b-IgA-IC、iC3b/C3dg-IgA-IC、C3b-IgM-IC和iC3b/C3dg-IgM-IC。发现活动性系统性红斑狼疮（SLE）或IgA肾病患者约四分之三的血浆样本中一种或多种类型的ICs呈阳性。IgA型ICs在自身免疫性疾病或肾脏疾病患者中非常常见。
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