Klangpetch Wannaporn, Noma Seiji, Igura Noriyuki, Shimoda Mitsuya
Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University.
Biocontrol Sci. 2013;18(4):199-203. doi: 10.4265/bio.18.199.
In order to understand the microbial inactivation mechanism of high-pressure carbonation (HPC), we examined the changes in the activity of the respiratory chain and DNA damage in Escherichia coli cells. HPC was performed under 1-6 MPa at 30°C for 1 min. The increase in CO2 pressure decreased the number of viable cells of E. coli, intracellular ATP, and intracellular NADH, and increased the number of apurinic/apyrimidinic sites. These results indicate that HPC has a detrimental effect on the functioning of the respiratory chain in E. coli and induces DNA damage, which could result in the death of the bacterial cells.
为了了解高压碳酸化（HPC）的微生物灭活机制，我们检测了大肠杆菌细胞中呼吸链活性和DNA损伤的变化。HPC在30°C、1-6 MPa压力下进行1分钟。二氧化碳压力的增加降低了大肠杆菌的活菌数、细胞内ATP和细胞内NADH，并增加了脱嘌呤/脱嘧啶位点的数量。这些结果表明，HPC对大肠杆菌呼吸链的功能有不利影响，并诱导DNA损伤，这可能导致细菌细胞死亡。
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