Haraguchi S, Kurakata S, Fujii T, Matsuo T, Yoshida T O
Cell Immunol. 1987 Apr 1;105(2):340-54. doi: 10.1016/0008-8749(87)90082-7.
The specificities of cytotoxic T lymphocytes (CTL) were studied for the analysis of CTL against tumor-specific cell surface antigen(s) (TSSA) of non-virus-producing tumor cells induced by the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV) in B10 congenic and recombinant mice. Eight CTL clones were established from immune spleen cells of B10.A(5R) mice. These clones demonstrated six patterns of cytotoxic reactivity in vitro: Two clones showed H-2 restriction in tumor cell lysis. Two other clones had the capacity to lyse syngeneic, H-2K-compatible B10 and H-2-incompatible B10.A(4R) tumor cells, but not YAC-1 cells. One clone had cytotoxic activity against syngeneic, H-2D-compatible B10.D2 tumor cells and YAC-1 cells, but not against H-2-incompatible tumor cells. One clone had cytotoxic activity against syngeneic and YAC-1 tumor cells, but not against either H-2-compatible or H-2-incompatible tumor cells. One clone had lytic activity to syngeneic, H-2-compatible, H-2-incompatible, and YAC-1 tumor cells. Another clone killed H-2-incompatible B10.A(4R) tumor and YAC-1 cells, but not syngeneic or H-2-compatible tumor cells. All these clones strongly expressed surface Thy-1.2 antigens, whereas the expression of Lyt-1.2 and Lyt-2.2 antigens was different from clone to clone. These results demonstrate heterogeneity of both lytic specificity and phenotype of CTL against RSV-induced mouse tumor cells, suggesting the existence of multiple antigenic sites on the RSV TSSA recognized by CTL populations.
在B10同源和重组小鼠中，研究了细胞毒性T淋巴细胞（CTL）的特异性，以分析针对由劳斯肉瘤病毒施密特-鲁平株（SR-RSV）诱导的非病毒产生肿瘤细胞的肿瘤特异性细胞表面抗原（TSSA）的CTL。从B10.A(5R)小鼠的免疫脾细胞中建立了8个CTL克隆。这些克隆在体外表现出六种细胞毒性反应模式：两个克隆在肿瘤细胞裂解中表现出H-2限制。另外两个克隆有能力裂解同基因、H-2K相容的B10和H-2不相容的B10.A(4R)肿瘤细胞，但不能裂解YAC-1细胞。一个克隆对同基因、H-2D相容的B10.D2肿瘤细胞和YAC-1细胞具有细胞毒性活性，但对H-2不相容的肿瘤细胞没有活性。一个克隆对同基因和YAC-1肿瘤细胞具有细胞毒性活性，但对H-2相容或H-2不相容的肿瘤细胞都没有活性。一个克隆对同基因、H-2相容、H-2不相容和YAC-1肿瘤细胞具有裂解活性。另一个克隆杀死H-2不相容的B10.A(4R)肿瘤细胞和YAC-1细胞，但不杀死同基因或H-2相容的肿瘤细胞。所有这些克隆都强烈表达表面Thy-1.2抗原，而Lyt-1.2和Lyt-2.2抗原的表达因克隆而异。这些结果证明了针对RSV诱导的小鼠肿瘤细胞的CTL的裂解特异性和表型的异质性，表明CTL群体识别的RSV TSSA上存在多个抗原位点。
