Benjamin D C, Herr J C, Sutherland W M, Woodward M P, DeCourcy K, Condon T P
Hybridoma. 1987 Apr;6(2):183-90. doi: 10.1089/hyb.1987.6.183.
A panel of monoclonal antibodies was raised against human serum albumin from fusions of BALB/c splenocytes and SP2/0-Ag14 murine myeloma cells. This panel was screened against purified albumins from 21 species including chimpanzee, gorilla, and orangutan. A monoclonal antibody (HSA-1) specific for human albumin was identified. The epitope recognized by HSA-1 was shown to be conserved in all human blood samples tested. A double antibody ELISA assay was developed using biotinylated HSA-1 as the specific probe for human albumin. This assay was capable of detecting as little as 30 nanograms or less albumin/ml. This assay was used to verify the presence of human albumin in blood, tissue extracts, and other body fluids. These results show that the HSA-1 monoclonal antibody can be used in determining the human origin of blood, tissue, and a variety of other body fluids.
利用BALB/c脾细胞与SP2/0-Ag14鼠骨髓瘤细胞融合产生了一组针对人血清白蛋白的单克隆抗体。该组抗体针对包括黑猩猩、大猩猩和猩猩在内的21种动物的纯化白蛋白进行筛选。鉴定出一种对人白蛋白具有特异性的单克隆抗体（HSA-1）。结果表明，HSA-1识别的表位在所有检测的人类血液样本中都是保守的。使用生物素化的HSA-1作为人白蛋白的特异性探针，开发了一种双抗体ELISA检测方法。该检测方法能够检测低至30纳克或更低浓度（每毫升）的白蛋白。该检测方法用于验证血液、组织提取物和其他体液中是否存在人白蛋白。这些结果表明，HSA-1单克隆抗体可用于确定血液、组织和多种其他体液的人类来源。
