新生仔猪心脏C型利钠肽基因表达及血浆浓度
Cardiac C-type natriuretic peptide gene expression and plasma concentrations in neonatal piglets.
作者信息
Hansen Lasse H, Smith Julie, Goetze Jens P
机构信息
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark.
Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, 9 Blegdamsvej, DK-2100 Copenhagen, Denmark; Department of Technology, Faculty of Health and Technology, Metropolitan University College, Sigurdsgade 26, DK-2200 Copenhagen, Denmark.
出版信息
Regul Pept. 2014 Jan 10;188:66-9. doi: 10.1016/j.regpep.2013.12.007. Epub 2013 Dec 27.
BACKGROUND
C-type natriuretic peptide (CNP) is a member of the natriuretic peptide family. Cardiac ANP and BNP expressions are firmly established, whereas CNP expression in the mammalian heart remains controversial. In the present report, we used a porcine model of the neonatal period with high expressions of cardiac ANP and BNP in order to elucidate the cardiac CNP expression profile.
METHODS
Plasma and cardiac tissue were obtained from newborn piglets during the first 72 h of life. The chamber-specific CNP mRNA contents were quantified by real-time PCR analysis. The proCNP concentrations in plasma and cardiac tissue extracts were quantified by a porcine-specific radioimmunoassay.
RESULTS
Cardiac CNP mRNA contents (n=24) were low compared to sites of known expression, where porcine seminal vesicle CNP mRNA contents were 200-fold higher. In addition, plasma proCNP concentrations in the newborn piglets (n=44) were exceedingly low compared to proANP concentrations (5.3 pmol/L (3.2-8.6) vs. 3438 pmol/L (2790-5418), p<0.0001). The proCNP concentrations in atrial tissue extracts were barely detectable (≤0.06 pmol/g) (n=2) compared to ventricular proANP (130 pmol/g (101-159)) and atrial proANP (12,303 pmol/g (10,623-15,412)).
CONCLUSION
Our data show that the heart is not a major source of circulating proCNP in neonatal piglets.
背景
C型利钠肽（CNP）是利钠肽家族的一员。心脏中ANP和BNP的表达已被明确证实，而哺乳动物心脏中CNP的表达仍存在争议。在本报告中，我们使用了新生期猪模型，该模型中心脏ANP和BNP表达较高，以阐明心脏CNP的表达谱。
方法
在新生仔猪出生后的头72小时内获取血浆和心脏组织。通过实时PCR分析定量心室特异性CNP mRNA含量。通过猪特异性放射免疫测定法定量血浆和心脏组织提取物中的proCNP浓度。
结果
与已知表达部位相比，心脏CNP mRNA含量（n = 24）较低，其中猪精囊CNP mRNA含量高200倍。此外，与proANP浓度相比，新生仔猪（n = 44）血浆中的proCNP浓度极低（5.3 pmol/L（3.2 - 8.6）对3438 pmol/L（2790 - 5418），p < 0.0001）。与心室proANP（130 pmol/g（101 - 159））和心房proANP（12,303 pmol/g（10,623 - 15,412））相比，心房组织提取物中的proCNP浓度几乎检测不到（≤0.06 pmol/g）（n = 2）。
结论
我们的数据表明，心脏不是新生仔猪循环proCNP的主要来源。
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