pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University, Frankfurt, Germany.
J Sep Sci. 2014 Mar;37(5):476-83. doi: 10.1002/jssc.201301173. Epub 2014 Jan 29.
LC-MS/MS has been applied for the rapid determination of the nucleoside analogue ribavirin in human plasma and red blood cells. The incorporation of ribavirin to the erythrocytes has been assayed after in vitro incubation of the cells at different concentrations of the antiviral drug. After protein precipitation, samples were injected into a C8 column, achieving a complete separation of ribavirin from the endogenous isobaric compound uridine. Calibration ranges varied from 10 to 10,000 ng/mL in plasma and from 0.2 to 200 ng/cell pellet in red blood cells. Precision and accuracy values were always below 10 and 13%, respectively, in all assayed matrices. Ribavirin was demonstrated to remain unchanged after short and long time storage. No matrix effects could be assessed for the analyzed matrices. The developed method has been fully validated. Monitoring of ribavirin concentration in red blood cells in addition to the classic plasma monitoring of the drug could help to explain its efficacy and safety profiles in patients.
LC-MS/MS 已被应用于快速测定人血浆和红细胞中的核苷类似物利巴韦林。在不同浓度抗病毒药物体外孵育后，测定利巴韦林向红细胞的掺入。经蛋白沉淀后，将样品注入 C8 柱，实现利巴韦林与内源性等排化合物尿苷的完全分离。在血浆中的校准范围为 10 至 10,000ng/mL，在红细胞中的细胞沉淀中为 0.2 至 200ng/细胞。在所有检测的基质中，精密度和准确度值始终分别低于 10%和 13%。证明利巴韦林在短时间和长时间储存后保持不变。对于分析的基质，无法评估基质效应。已对所开发的方法进行了全面验证。除了对药物进行经典的血浆监测外，监测红细胞中的利巴韦林浓度有助于解释其在患者中的疗效和安全性特征。
