Zhao Cheng-Xiao, Liu Ming, Wang Jian-Ye, Xu Yong, Wei Dong, Yang Kuo, Yang Ze
The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Chinese Ministry of Health, Beijing, China E-mail :
Asian Pac J Cancer Prev. 2014 Jan;14(11):6733-8. doi: 10.7314/apjcp.2013.14.11.6733.
Multiple genetic studies have confirmed association of 8q24 variants with susceptibility to prostate cancer (PCa). As PCa risk SNPs may also influence disease outcome, we studied here eight 8q24 risk alleles, and evaluated their role in PCa clinical covariates in northern Chinese men. Blood samples and clinical information were collected from ethnically Chinese men from Northern China with histologically-confirmed PCa (n=289) and from age-matched normal controls (n=288). Eight 8q24 SNPs were genotyped by polymerase chain reaction- high-resolution melting analysis in 577 subjects. We examined the prevalence distribution of 8q24 risk alleles and analyzed the associations between the risk allele and PCa and clinical covariates to infer their impact on aggressive PCa. Three of the eight SNPs were associated with PCa risk in northern Chinese men, including rs16901966 (OR 1.31, 95% CI 1.01-1.70, p=0.042), rs1447295 (OR 1.47, 95% CI 1.09-1.98, p=0.011) and rs10090154 (OR 1.55, 95% CI 1.14-2.12, p=0.005). Haplotype analysis based association with the risk alleles revealed significant differences between cases and controls (OR 1.43, 95%CI 0.99-2.06, p=0.049). The risk alleles rs16901966, rs1447295 and rs10090154 were associated with age at diagnosis and tumor stage as compared with controls, while rs16901966 was associated with aggressive PCa (OR 1.43, 95% CI 1.01-2.03, p=0.042). The evidence for 8q24 SNPs with PCa risk in northern Chinese men showed rs16901966, rs1447295 and rs10090154 at 8q24 (region 1, region 2) to be strongly associated with PCa and clinical covariates. The three SNPs at 8q24 could be PCa susceptible genetic markers in northern Chinese men.
多项基因研究已证实8q24变异与前列腺癌（PCa）易感性相关。由于PCa风险单核苷酸多态性（SNP）也可能影响疾病预后，我们在此研究了8个8q24风险等位基因，并评估了它们在中国北方男性PCa临床协变量中的作用。从中国北方经组织学确诊为PCa的汉族男性（n = 289）和年龄匹配的正常对照者（n = 288）中收集血液样本和临床信息。通过聚合酶链反应-高分辨率熔解分析对577名受试者的8个8q24 SNP进行基因分型。我们检查了8q24风险等位基因的流行分布，并分析了风险等位基因与PCa及临床协变量之间的关联，以推断它们对侵袭性PCa的影响。8个SNP中的3个与中国北方男性的PCa风险相关，包括rs16901966（比值比[OR] 1.31，95%可信区间[CI] 1.01 - 1.70，p = 0.042）、rs1447295（OR 1.47，95% CI 1.09 - 1.98，p = 0.011）和rs10090154（OR 1.55，95% CI 1.14 - 2.12，p = 0.005）。基于单倍型分析与风险等位基因的关联显示病例组和对照组之间存在显著差异（OR 1.43，95% CI 0.99 - 2.06，p = 0.049）。与对照组相比，风险等位基因rs16901966、rs1447295和rs10090154与诊断年龄和肿瘤分期相关，而rs16901966与侵袭性PCa相关（OR 1.43，95% CI 1.01 - 2.03，p = 0.042）。中国北方男性中8q24 SNP与PCa风险的证据表明，8q24（区域1、区域2）的rs16901966、rs1447295和rs10090154与PCa及临床协变量密切相关。8q24的这3个SNP可能是中国北方男性PCa易感性的遗传标记。
