Department of Urology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Research Institute of Medical Sciences, Chonnam National University, Gwangju 501-757, Korea.
Department of Physiology, Chonnam National University Medical School, Gwangju 501-757, Korea. ; Research Institute of Medical Sciences, Chonnam National University, Gwangju 501-757, Korea.
Korean J Physiol Pharmacol. 2013 Dec;17(6):531-6. doi: 10.4196/kjpp.2013.17.6.531. Epub 2013 Dec 16.
Interstitial cells of Cajal (ICCs) from the urinary bladder regulate detrusor smooth muscle activities. We cultured ICCs from the urinary bladder of mice and performed patch clamp and intracellular Ca(2+) ([Ca(2+)]i) imaging to investigate whether cultured ICCs can be a valuable tool for cellular functional studies. The cultured ICCs displayed two types of spontaneous electrical activities which are similar to those recorded in intact bladder tissues. Spontaneous electrical activities of cultured ICCs were nifedipine-sensitive. Carbachol and ATP, both excitatory neurotransmitters in the urinary bladder, depolarized the membrane and increased the frequency of spike potentials. Carbachol increased [Ca(2+)]i oscillations and basal Ca(2+) levels, which were blocked by atropine. These results suggest that cultured ICCs from the urinary bladder retain rhythmic phenotypes similar to the spontaneous electrical activities recorded from the intact urinary bladder. Therefore, we suggest that cultured ICCs from the urinary bladder may be useful for cellular and molecular studies of ICCs.
Cajal 间质细胞（ICCs）来源于膀胱，调节逼尿肌平滑肌的活动。我们从小鼠的膀胱中培养 ICCs，并进行膜片钳和细胞内 Ca(2+)([Ca(2+)]i)成像，以研究培养的 ICCs 是否可以作为细胞功能研究的有价值工具。培养的 ICCs 显示出两种类似于在完整膀胱组织中记录到的自发性电活动。培养的 ICCs 的自发性电活动对硝苯地平敏感。在膀胱中作为兴奋性神经递质的乙酰胆碱和 ATP 使膜去极化并增加尖峰电位的频率。乙酰胆碱增加了[Ca(2+)]i 振荡和基础 Ca(2+)水平，该作用被阿托品阻断。这些结果表明，来源于膀胱的培养 ICCs 保留了类似于从完整膀胱中记录到的自发性电活动的节律表型。因此，我们认为，来源于膀胱的培养 ICCs 可能对 ICCs 的细胞和分子研究有用。
