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草本石南的酚类含量、抗菌及抗氧化活性

Phenolic content, antibacterial and antioxidant activities of Erica herbacea L.

作者信息

Vucić Dragana M, Petković Miroslav R, Rodić-Grabovac Branka B, Stefanović Olgica D, Vasić Sava M, Comić Ljiljana R

机构信息

Department of Microbiology, Medical Faculty, University of Banja Luka, Save Mrkalja 14, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina.

Department of Organic Chemistry, Faculty of Technology, University of Banja Luka, Bulevar Stepe Stepanovića 73, 78000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina.

出版信息

Acta Pol Pharm. 2013 Nov-Dec;70(6):1021-6.

Abstract

Antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of Erica herbacea L. were studied. In vitro antibacterial activity of the extracts was determined by macrodilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. Testing was performed on 30 clinical isolates, including different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris. The values for MIC were in the range from 2.5 mg/mL to 40 mg/mL. The most sensitive bacterial strains were Proteus vulgaris strains. The aqueous extract from E. herbacea was found the most active. The total phenolic content was determined using Folin-Ciocalteu reagent and ranged between 14.98 and 119.88 mg GA/g. The concentration of flavonoids in extracts was determined using spectrophotometric method with aluminium chloride and obtained results varied from 16.19 to 26.90 mg RU/g. Antioxidant activity was monitored spectrophotometrically using DPPH reagent. The highest capacity to neutralize DPPH radicals was found in the aqueous extract from E. herbacea. The results of the total phenolic content determination of the examined extracts indicate that E. herbacea extracts are a rich source of phenolic compounds and also possess a significant antioxidant activity and moderate antibacterial activity.

摘要

对草本石南(Erica herbacea L.)叶和花的水提取物、乙醇提取物和乙酸乙酯提取物的抗菌和抗氧化活性、总酚和黄酮浓度进行了研究。采用宏观稀释法测定提取物的体外抗菌活性。测定了最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。对30株临床分离株进行了检测,包括不同菌株的大肠杆菌、粪肠球菌和普通变形杆菌。MIC值范围为2.5mg/mL至40mg/mL。最敏感的细菌菌株是普通变形杆菌菌株。发现草本石南的水提取物活性最强。使用福林-酚试剂测定总酚含量,范围在14.98至119.88mg GA/g之间。使用氯化铝分光光度法测定提取物中的黄酮浓度,得到的结果在16.19至26.90mg RU/g之间变化。使用DPPH试剂通过分光光度法监测抗氧化活性。发现草本石南的水提取物中和DPPH自由基的能力最强。所检测提取物的总酚含量测定结果表明,草本石南提取物是酚类化合物的丰富来源,还具有显著的抗氧化活性和适度的抗菌活性。

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