Institute of Environmental Science and Technology, Zhejiang University, Hangzhou 310058, China.
Ningda Inspection and Technology Co. Ltd., Hangzhou 310004, China.
Chemosphere. 2014 Apr;101:77-85. doi: 10.1016/j.chemosphere.2013.12.030. Epub 2014 Jan 4.
A combined process consisting of ex situ nitrification and in situ denitrification in landfill refuse was studied in pilot scale for nitrogen removal from municipal landfill leachate. The results showed that above 80% of partial nitrification ratio and an average COD loading rate of 1.50 kg m(-3) d(-1) were steadily maintained under DO concentrations of 1.0-1.7 mg L(-1) in the aerobic reactor. Quantitative PCR results indicated that nitrite-oxidizing bacteria being sensitive to DO fluctuations lead to partial nitrification when free ammonia inhibition was weak. Nitrified landfill leachate could be denitrified in the landfill bioreactor with maximum total oxidizing nitrogen removal rate of 67.2 g N t(-1) TSwaste d(-1). Clone and sequencing analysis of denitrifying bacterial nirS gene inferred that heterotrophic denitrifier Azoarcus tolulyticu was the primary nitrogen converter in the landfill bioreactor. The obtained results will provide valuable information for optimizing the design and operation of a landfill bioreactor.
采用中试规模，研究了垃圾填埋渗滤液中脱氮的反硝化和硝化组合工艺。结果表明，在好氧反应器中 DO 浓度为 1.0-1.7mg/L 时，可稳定保持 80%以上的部分硝化比和 1.50kg m(-3) d(-1)的平均 COD 负荷率。定量 PCR 结果表明，当游离氨抑制较弱时，对 DO 波动敏感的亚硝酸盐氧化菌会导致部分硝化。硝化的垃圾渗滤液可在垃圾生物反应器中反硝化，最大总氧化氮去除率为 67.2g N t(-1) TSwaste d(-1)。反硝化细菌nirS 基因的克隆和测序分析表明，异养反硝化菌 Azoarcus tolulyticu 是垃圾生物反应器中的主要氮转化菌。所得结果将为优化垃圾生物反应器的设计和运行提供有价值的信息。
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