一种使用抗体偶联的球形介孔二氧化硅与固定化酶的高灵敏度免疫测定法。
A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes.
作者信息
Eum Ji Young, Hwang Sang Youn, Ju Youngjun, Shim Jong Min, Piao Yunxian, Lee Jinwoo, Kim Hak-Sung, Kim Jungbae
机构信息
Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
出版信息
Chem Commun (Camb). 2014 Apr 7;50(27):3546-8. doi: 10.1039/c3cc48044e.
A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.
通过使用结合抗体的球形介孔二氧化硅与固定化酶，开发了一种高灵敏度免疫测定法。与传统酶联免疫吸附测定（ELISA）相比，更高的酶/抗体比例提高了灵敏度和动态范围。特别是，使用球形介孔二氧化硅可实现的检测限（LOD），其灵敏度比使用无定形二氧化硅的ELISA高20倍。
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