[一个假设的基因对ssl2138和sll11092在集胞藻PCC 6803染色体上构成一个功能性的毒素-抗毒素系统]
[A hypothetical gene pair, ssl2138 and sll11092, constitutes a functional TA system on the chromosome of Synechocystis PCC 6803].
作者信息
Ning Degang, Zhao Weifei, Qian Yaru
机构信息
School of the Environment, Jiangsu University, 212013 Zhenjiang, China.
出版信息
Wei Sheng Wu Xue Bao. 2013 Oct 4;53(10):1043-9.
OBJECTIVE
We characterized the hypothetical gene pair ssl2138 and sll1092 that constitute a vapBC-family toxin-antitoxin (TA) system on the chromosome of Synechocystis PCC 6803.
METHODS
An RT-PCR analysis was conducted to confirm the co-transcription of this gene pair, a selection-expression system was constructed to demonstrate the effect of their encoded proteins on E. coli growth. To validate interaction between these two proteins, an affinity capture analysis was performed.
RESULTS
ssl2138 and sll1092 were co-transcribed under normal growth conditions. Ectopic expression of sll1092 inhibited the growth of E. coli, which could be overcome by simultaneous or subsequent expression of ssl2138. Both proteins Ssl2138 and Sll1092 in vivo formed a protein complex due to their specific interaction.
CONCLUSION
ssl2138 and sll1092 constitute a functional vapBC-family TA system of Synechocystis PCC 6803.
目的
我们对构成集胞藻PCC 6803染色体上一个vapBC家族毒素-抗毒素（TA）系统的假定基因对ssl2138和sll1092进行了表征。
方法
进行逆转录聚合酶链反应（RT-PCR）分析以确认该基因对的共转录，构建了一个选择表达系统以证明其编码蛋白对大肠杆菌生长的影响。为验证这两种蛋白之间的相互作用，进行了亲和捕获分析。
结果
ssl2138和sll1092在正常生长条件下共转录。sll1092的异位表达抑制了大肠杆菌的生长，而ssl2138的同时或后续表达可克服这种抑制。由于Ssl2138和Sll1092这两种蛋白之间的特异性相互作用，它们在体内形成了一个蛋白复合物。
结论
ssl2138和sll1092构成了集胞藻PCC 6803的一个功能性vapBC家族TA系统。
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