Department of Entomology, Fisheries, and Wildlife, University of Minnesota, 55108, St. Paul, Minnesota.
J Chem Ecol. 1982 Jan;8(1):83-94. doi: 10.1007/BF00984007.
The existence of a female-produced sex pheromone in the yellowheaded spruce sawfly,Pikonema alaskensis (Rohwer) (Hymenoptera: Tenthredinidae) was demonstrated by field and greenhouse bioassays. Virgin females, their empty cocoons (with which they were confined during handling procedures), and the hexane extract of these cocoons were attractive in the field. The only Florisil fraction of this extract consistently attractive by itself was that eluted with hexane, but three, more polar fractions (eluted with 5%, 25%, and 50% ether in hexane) each synergized the hexane fraction, increasing bioassay responses 10-30 times. Fractions derived directly from virgin females yielded comparable results. The greenhouse data corroborated the field data, except that the 5% ether-hexane fraction, while very synergistic in the field, was consistently inactive in the greenhouse.
通过野外和温室生物测定证明，黄头云杉锯蝇（Pikonema alaskensis（Rohwer））（膜翅目：叶蜂科）的雌性产生性信息素。处女雌性、它们的空茧（在处理过程中用空茧困住）以及这些茧的己烷提取物在野外具有吸引力。该提取物中唯一的 Florisil 馏分本身就具有吸引力，但三个极性更大的馏分（用 5%、25%和 50%乙醚在己烷中洗脱）都协同作用于己烷馏分，使生物测定反应增加 10-30 倍。直接从处女雌性获得的馏分产生了可比的结果。温室数据与野外数据相吻合，只是 5%乙醚-己烷馏分在野外非常协同，但在温室中始终不活跃。
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