Srabovic Nahida, Mujagic Zlata, Mujanovic-Mustedanagic Jasminka, Softic Adaleta, Muminovic Zdeno, Rifatbegovic Adi, Begic Lejla
Department of Biochemistry, University of Tuzla, 75 000 Tuzla, Bosnia And Herzegovina.
Policlinic of Laboratory Diagnostics, Department of Pathology, University Clinical Centre of Tuzla, 75 000 Tuzla, Bosnia And Herzegovina.
Int J Breast Cancer. 2013;2013:746749. doi: 10.1155/2013/746749. Epub 2013 Dec 12.
VEGF-A is the most potent angiogenic factor in tumour angiogenesis. Its effects are mediated via two receptors VEGFR-1 and VEGFR-2. Primary aim of our study was to examine the expression of VEGFR-1 in breast cancer and its correlation to VEGF expression, lymph node status, tumour size, histological grade, and hormone receptor status. To examine the VEGFR-1 and VEGF expressions in tumour and surrounding tissue of 51 breast cancer patients, and in healthy breast tissue of 30 benign breast diseases patients, we used three-step immunohistochemical staining. VEGFR-1 and VEGF expressions were significantly increased in breast cancer tumour in relation to surrounding tissue (P < 0.01), and the VEGF expression was significantly increased in lymph node positive breast cancer patients (P < 0.01). VEGFR-1 and VEGF expressions were significantly higher in breast cancer tumour compared with healthy breast tissue (P < 0.01). Significant correlation between VEGF and VEGFR-1 expressions was found (P < 0.05). No significant correlations between VEGF and VEGFR-1 expressions and tumour size, histological grade, and hormone receptor status were found. Increased expression of VEGFR-1 and VEGF in breast cancer tumour and significant correlation between these proteins suggest the possible role of VEGF/VEGFR-1 signalization in breast cancer development, although VEGFR-1 potential prognostic value was not confirmed.
血管内皮生长因子A（VEGF-A）是肿瘤血管生成中最有效的血管生成因子。其作用通过两种受体VEGFR-1和VEGFR-2介导。我们研究的主要目的是检测VEGFR-1在乳腺癌中的表达及其与VEGF表达、淋巴结状态、肿瘤大小、组织学分级和激素受体状态的相关性。为检测51例乳腺癌患者肿瘤及周围组织以及30例乳腺良性疾病患者健康乳腺组织中VEGFR-1和VEGF的表达，我们采用了三步免疫组织化学染色法。与周围组织相比，乳腺癌肿瘤中VEGFR-1和VEGF的表达显著增加（P<0.01），且淋巴结阳性乳腺癌患者中VEGF的表达显著增加（P<0.01）。与健康乳腺组织相比，乳腺癌肿瘤中VEGFR-1和VEGF的表达显著更高（P<0.01）。发现VEGF与VEGFR-1的表达之间存在显著相关性（P<0.05）。未发现VEGF和VEGFR-1的表达与肿瘤大小、组织学分级和激素受体状态之间存在显著相关性。乳腺癌肿瘤中VEGFR-1和VEGF表达增加以及这些蛋白之间的显著相关性表明VEGF/VEGFR-1信号传导在乳腺癌发展中可能发挥作用，尽管VEGFR-1的潜在预后价值尚未得到证实。
