RAGE 基因多态性与印度 2 型糖尿病患者血管并发症的关系。
Association of RAGE gene polymorphism with vascular complications in Indian type 2 diabetes mellitus patients.
机构信息
Biochemistry and Immunology Laboratory, Department of Biochemistry, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India.
Department of Medicine, University College of Medical Sciences (University of Delhi) and G.T.B. Hospital, Dilshad Garden, Delhi 110095, India.
出版信息
Diabetes Res Clin Pract. 2014 Mar;103(3):474-81. doi: 10.1016/j.diabres.2013.12.004. Epub 2013 Dec 25.
AIMS
The study was designed to evaluate the association of -374T/A and -429T/C polymorphism in the promoter region and Gly82Ser polymorphism in exon 3 region of RAGE gene with diabetic vascular complications in Indian population.
METHODS
We screened 603 subjects which includes 176 healthy controls, 140 type 2 diabetes mellitus (T2DM) subjects without any vascular complications (DM), 152 T2DM subjects with microvascular complications (DM-micro) and 135 T2DM subjects with macrovascular complications (DM-macro) for -374T/A, -429T/C and Gly82Ser polymorphisms of RAGE gene. DNA isolated from the enrolled subjects were genotyped by PCR-RFLP. Logistic regression analysis was used to evaluate the association of single nucleotide polymorphisms (SNPs).
RESULTS
The -429 T/C and Gly82Ser RAGE polymorphisms were found to be significantly associated with the development of macrovascular and microvascular complications, respectively, in T2DM subjects while -374A allele showed reduced risk towards the development of macrovascular complications. Further, -429T/C, -374T/A and Gly82Ser haplotype analysis revealed association of CTG haplotype with development of macrovascular complications while haplotype TAG was observed to be significantly protective towards development of macrovascular complications in T2DM subjects (OR=0.617, p=0.0202).
CONCLUSIONS
Our data indicates significant association of RAGE SNPs and haplotypes with vascular complications in North Indian T2DM subjects.
目的
本研究旨在评估 RAGE 基因启动子区域的-374T/A 和-429T/C 多态性以及外显子 3 区域的 Gly82Ser 多态性与印度人群糖尿病血管并发症的关系。
方法
我们筛选了 603 名受试者，包括 176 名健康对照者、140 名无血管并发症的 2 型糖尿病（T2DM）患者（DM）、152 名伴有微血管并发症的 T2DM 患者（DM-micro）和 135 名伴有大血管并发症的 T2DM 患者（DM-macro），用于 RAGE 基因的-374T/A、-429T/C 和 Gly82Ser 多态性。从入组受试者中提取的 DNA 通过 PCR-RFLP 进行基因分型。采用逻辑回归分析评估单核苷酸多态性（SNP）的相关性。
结果
-429T/C 和 Gly82Ser RAGE 多态性与 T2DM 患者大血管和微血管并发症的发生显著相关，而-374A 等位基因则降低了大血管并发症的发病风险。此外，-429T/C、-374T/A 和 Gly82Ser 单体型分析显示 CTG 单体型与大血管并发症的发生相关，而 TAG 单体型则观察到对 T2DM 患者大血管并发症的发生具有显著保护作用（OR=0.617，p=0.0202）。
结论
我们的数据表明，RAGE SNP 和单体型与北印度 T2DM 患者的血管并发症显著相关。
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