鉴定多粘类芽孢杆菌和粘质沙雷氏菌中的结构固氮（nif）基因。

Identification of structural nitrogen-fixation (nif) genes in Bacillus polymyxa and Bacillus macerans.

机构信息

, .

出版信息

World J Microbiol Biotechnol. 1993 May;9(3):387-9. doi: 10.1007/BF00383088.

PMID:24420051

Abstract

Some naturally occurring Nif(+) and Nif(-)uperscript> strains of Bacillus polymyxa and Bacillus macerans were tested for DNA homology to the Klebsiella pneumoniae nif genes by Southern hybridization. Genomic DNA from all Nif(+) strains carried homologous sequences only to structural nif genes. The homology detected was limited to nifH and nifD. The hybridization pattern observed suggested that both genes are contiguous on the chromosome of the Bacillus strains. No homology was found between the genomic DNA from Nif(-)uperscript> strains and the K. pneumoniae nif genes.

摘要

一些天然存在的 Nif(+) 和 Nif(-) 超支>芽孢杆菌和粘液芽孢杆菌菌株通过 Southern 杂交试验来测试其与肺炎克雷伯氏菌 nif 基因的 DNA 同源性。所有 Nif(+) 菌株的基因组 DNA 仅携带与结构 nif 基因同源的序列。检测到的同源性仅限于 nifH 和 nifD。观察到的杂交模式表明，这两个基因在芽孢杆菌菌株的染色体上是连续的。Nif(-)超支>菌株的基因组 DNA 与肺炎克雷伯氏菌 nif 基因之间没有发现同源性。