Stamatoyannopoulos G, Constantoulakis P, Brice M, Kurachi S, Papayannopoulou T
Dev Biol. 1987 Sep;123(1):191-7. doi: 10.1016/0012-1606(87)90441-6.
The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yolk sac (primitive) erythropoiesis, in addition to embryonic chains, produced fetal and adult globins and that cells of the definitive (liver) erythropoiesis, in addition to fetal and adult globins, produce embryonic globins. That embryonic, fetal, and adult globins were coexpressed by cells of the same lineage was documented by analysis of globin chains in single BFUe colonies: all 67 yolk sac-origin BFUe colonies and 42 of 43 liver-origin BFUe colonies synthesized epsilon-, gamma-, and beta-chains. These data showed that during the switch from embryonic to adult globin formation, embryonic and definitive globin chains are coexpressed in the primitive, as well as in the definitive, erythroid cells. Such results are compatible with the postulate that the switch from embryonic to fetal globin synthesis represents a time-dependent change in programs of progenitor cells rather than a change in hemopoietic cell lineages.
通过对35至56日龄胚胎红系细胞中珠蛋白表达的研究，对人类胚胎向胎儿珠蛋白形成转换的细胞调控进行了调查。对体内和红系祖细胞（爆式集落形成单位，BFUe）培养物中合成的珠蛋白的分析表明，卵黄囊（原始）红细胞生成的细胞除了产生胚胎链外，还产生胎儿和成人珠蛋白，而定型（肝脏）红细胞生成的细胞除了产生胎儿和成人珠蛋白外，还产生胚胎珠蛋白。通过对单个BFUe集落中的珠蛋白链进行分析，证明了胚胎、胎儿和成人珠蛋白由同一谱系的细胞共同表达：所有67个卵黄囊来源的BFUe集落和43个肝脏来源的BFUe集落中的42个都合成了ε-、γ-和β-链。这些数据表明，在从胚胎向成人珠蛋白形成的转换过程中，胚胎和定型珠蛋白链在原始和定型红系细胞中共同表达。这些结果与以下假设相符，即从胚胎向胎儿珠蛋白合成的转换代表祖细胞程序中随时间的变化，而不是造血细胞谱系的变化。
