Cotton Quality Laboratories, U.S. Department of Agriculture, Agricultural Research Service, Department of Plant and Soil Science, University of Tennessee, 37916, Knoxville, TN, USA.
Planta. 1977 Jan;137(2):113-7. doi: 10.1007/BF00387547.
A convenient and reliable method for culturing cotton embryos is needed to obtain interspecific hybrids of this genus. C.A. Beasley and I.P. Ting (Amer. J. Bot. 60, 130, 1973) developed a phytohormone-supplemented medium (BTP) upon which the growth of ovules was similar that of in situ ovules. This medium was examined for in-ovulo embryo culture. Although good ovule growth occurred on BTP no embryos developed to maturity. However, when the medium was supplemented with NH 4 (+) , more than 50% of the ovules produced mature embryos, and many of these germinated precociously after 8-10 weeks of culture. After germination seedlings were established on a separate medium designed to give balanced root and shoot growth. Subsequently young plants could be transferred to pots for greenhouse culture.
需要一种方便可靠的棉花胚胎培养方法，以获得该属的种间杂种。C.A. Beasley 和 I.P. Ting（《美国植物学杂志》60, 130, 1973）在补充植物激素的培养基（BTP）上培养胚珠，其生长类似于原位胚珠。本文研究了该培养基对胚珠内胚胎培养的作用。尽管 BTP 上的胚珠生长良好，但没有胚胎发育成熟。然而，当培养基中补充 NH 4 (+) 时，超过 50%的胚珠产生成熟胚胎，其中许多在培养 8-10 周后提前发芽。发芽后，幼苗在单独的培养基上建立，以实现均衡的根和芽生长。随后，将幼小植株移植到温室培养的盆中。
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