根瘤菌属 etli 染色体基因的特性，该基因对菜豆属植物的根瘤发育是必需的。

Characterization of a Rhizobium etli chromosomal gene required for nodule development on Phaseolus vulgaris L.

机构信息

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出版信息

World J Microbiol Biotechnol. 1994 Sep;10(5):583-9. doi: 10.1007/BF00367673.

Abstract

A chromosomal gene, required for nodule development on Phaseolus bean, was characterized from Rhizobium etli strain TAL182. MLC640 is a Tn5 insertion mutant of TAL182 which shows decreased motility in soft TY agar and is defective in nodule development. The site of Tn5 insertion in MLC640 mapped to a 3.6-kb EcoRI chromosomal fragment. The 3.6-kb fragment was subcloned from the cosmid pUHR80 which complemented MLC640. Further subcloning and site-directed Tn5 mutagenesis localized the gene for nodule development to a 1.7-kb region within the 3.6-kb EcoRI fragment. Southern hybridization using the 3.6-kb EcoRI fragment as the probe against genomic DNA of several Rhizobium spp. indicated that this gene is conserved in different rhizobia.

摘要

从 Rhizobium etli 菌株 TAL182 中鉴定出一个豆科植物结瘤发育所必需的染色体基因。MLC640 是 TAL182 的 Tn5 插入突变体，在软 TY 琼脂中运动性降低，并且结瘤发育缺陷。MLC640 中 Tn5 插入的位点映射到一个 3.6kb 的 EcoRI 染色体片段。该 3.6kb 片段从小 cosmid pUHR80 中克隆，该 cosmid 补充了 MLC640。进一步的亚克隆和定点 Tn5 诱变将结瘤发育基因定位在 3.6kb EcoRI 片段内的 1.7kb 区域。用 3.6kb EcoRI 片段作为探针，对几种 Rhizobium spp. 的基因组 DNA 进行 Southern 杂交，表明该基因在不同的根瘤菌中是保守的。