耐盐性相思根瘤菌的分离与筛选。
Selection of salt-tolerant Rhizobium isolates of Acacia nilotica.
机构信息
Microbiology & Molecular Genetics Unit, Tata Energy Research Institute, India Habitat Centre, 110 003, Lodhi Road, New Delhi, India.
出版信息
World J Microbiol Biotechnol. 1994 Nov;10(6):637-9. doi: 10.1007/BF00327949.
Among 35 Rhizobium isolates of Acacia nilotica, from different agro-climatic zones, two, ANG4 and ANG5, tolerated up to 850 mM NaCl and one, ANG3, was sensitive to NaCl above 250 mM. Nodulation and nitrogenase activity of the three isolates decreased with increasing concentration of salt up to 150 mM. Nodulation by ANG3 was 15% at 75 mM NaCl and nil at 100 mM. With ANG4 and ANG5, nodulation was only slightly decreased at 150 mM NaCl. Nitrogenase activity associated with plants inoculated with ANG3 was halved at 25 mM NaCl compared with salt-free controls, whereas isolates ANG4 and ANG5 retained 25% and 15% activity, respectively, even at 100 mM NaCl. Salt-tolerant Rhizobium isolates can therefore nodulate and fix N2 in saline soils.
在来自不同农业气候带的 35 株非洲相思根瘤菌（Acacia nilotica）分离株中，有两株（ANG4 和 ANG5）能耐受高达 850 mM 的 NaCl，一株（ANG3）对超过 250 mM 的 NaCl 敏感。三种分离株的结瘤和固氮酶活性随着盐浓度的增加而下降，最高可达 150 mM。ANG3 在 75 mM NaCl 时的结瘤率为 15%，在 100 mM NaCl 时则为零。ANG4 和 ANG5 在 150 mM NaCl 时结瘤率仅略有下降。与无盐对照相比，接种 ANG3 的植物的固氮酶活性在 25 mM NaCl 时减半，而分离株 ANG4 和 ANG5 分别在 100 mM NaCl 时保留了 25%和 15%的活性。因此，耐盐根瘤菌分离株可以在盐渍土壤中结瘤和固定 N2。
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