Cell Physiology Laboratory, Royal Botanic Gardens, Kew, Richmond, Surrey, UK.
Theor Appl Genet. 1974 Jan;44(3):133-7. doi: 10.1007/BF02981926.
During maturation of the pollen in species ofIberis (Cruciferae) materials from the anther tapetum are transferred to cavities of the exine. These sporophytically-derived materials are responsible for the rejection response induced in the stigmatic papillae within 4-6 h. following incompatible pollination, a response which leads to the occlusion of the stigma papillae by the polysaccharide, callose. The rejection reaction can be induced with agar or agarose gels into which pollen-wall materials have been allowed to diffuse, and also with isolated fragments of the tapetum itself taken from anthers of incompatible plants before transfer of its contents to the pollen. Partial fractionation of the exine-held materials using thin-layer gel filtration suggests that the incompatibility reactions are mediated by one or more "recognition" proteins or glycoproteins in a group with molecular weights in the range 10,000-25,000 daltons.The rejection response has been found to be a rapid and sensitive assay for incompatibility in several Cruciferae.
在 Iberis（十字花科）物种的花粉成熟过程中，来自花药绒毡层的物质被转移到外壁的腔室中。这些孢子衍生的物质负责在不亲和授粉后 4-6 小时内诱导柱头乳突中的排斥反应，这一反应导致柱头乳突被多糖、胼胝质封闭。排斥反应可以用琼脂或琼脂糖凝胶诱导，花粉壁物质可以在其中扩散，也可以用取自不亲和植物花药的绒毡层本身的分离片段诱导，前提是先将其内容物转移到花粉中。使用薄层凝胶过滤对外壁结合物质进行部分分级表明，不亲和反应是由分子量在 10,000-25,000 道尔顿范围内的一个或多个“识别”蛋白或糖蛋白介导的。在几种十字花科植物中，排斥反应已被发现是一种快速而敏感的不亲和性测定方法。
