Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, Jiangsu Province, China,
Bioprocess Biosyst Eng. 2014 Jun;37(6):1185-92. doi: 10.1007/s00449-013-1089-x. Epub 2014 Jan 16.
Biodegradation of pyridine by a novel bacterial strain, Rhizobium sp. NJUST18, was studied in batch experiments over a wide concentration range (from 100 to 1,000 mg l(-1)). Pyridine inhibited both growth of Rhizobium sp. NJUST18 and biodegradation of pyridine. The Haldane model could be fitted to the growth kinetics data well with the kinetic constants μ* = 0.1473 h(-1), K s = 793.97 mg l(-1), K i = 268.60 mg l(-1) and S m = 461.80 mg l(-1). The true μ max, calculated from μ*, was found to be 0.0332 h(-1). Yield coefficient Y X/S depended on S i and reached a maximum of 0.51 g g(-1) at S i of 600 mg l(-1). V max was calculated by fitting the pyridine consumption data with the Gompertz model. V max increased with initial pyridine concentration up to 14.809 mg l(-1) h(-1). The q S values, calculated from [Formula: see text], were fitted with the Haldane equation, yielding q Smax = 0.1212 g g(-1) h(-1) and q* = 0.3874 g g(-1) h(-1) at S m' = 507.83 mg l(-1), K s' = 558.03 mg l(-1), and K i' = 462.15 mg l(-1). Inhibition constants for growth and degradation rate value were in the same range. Compared with other pyridine degraders, μ max and S m obtained for Rhizobium sp. NJUST18 were relatively high. High K i and K i' values and extremely high K s and K s' values indicated that NJUST18 was able to grow on pyridine within a wide concentration range, especially at relatively high concentrations.
利用一株新的细菌菌株——根瘤菌 sp. NJUST18，在较宽的浓度范围内（100-1000mg/L）对吡啶进行了生物降解研究。吡啶既抑制了根瘤菌 sp. NJUST18 的生长，也抑制了吡啶的生物降解。Haldane 模型可以很好地拟合生长动力学数据，动力学常数 μ*=0.1473h(-1)、Ks=793.97mg/L、Ki=268.60mg/L 和 Sm=461.80mg/L。从 μ计算出的真实 μmax 为 0.0332h(-1)。Yield coefficient Y X/S 取决于 Si，并在 Si 为 600mg/L 时达到最大值 0.51g/g。通过拟合吡啶消耗数据与 Gompertz 模型，计算 V max。V max 随初始吡啶浓度增加，在 14.809mg/L h(-1)时达到最大值。根据 [Formula: see text] 计算的 q S 值，用 Haldane 方程拟合，得到 q Smax=0.1212g/g h(-1)和 q=0.3874g/g h(-1)，在 Sm'=507.83mg/L、Ks'=558.03mg/L 和 Ki'=462.15mg/L 时。生长和降解速率值的抑制常数在同一范围内。与其他吡啶降解菌相比，根瘤菌 sp. NJUST18 获得的 μmax 和 Sm 较高。高 Ki 和 Ki'值以及极高的 Ks 和 Ks'值表明，NJUST18 能够在较宽的浓度范围内，尤其是在相对较高的浓度下，利用吡啶生长。
