Grimwade J E, Cason E B, Beerman T A
Nucleic Acids Res. 1987 Aug 25;15(16):6315-29. doi: 10.1093/nar/15.16.6315.
The sites of bleomycin-induced cleavage of purified and intracellular simian virus 40 DNA were examined. Breaks in purified DNA were mapped to several discrete sites that were distributed throughout the viral genome, but were not associated with a common genetic element. Double-stranded breaks were made in positions of the first single-stranded nick, and regions of cuts were unaffected by changes in DNA superhelicity. Bleomycin cut intracellular chromosomes at the same sites that were cleaved in purified DNA. These results indicate that SV40 DNA contains DNA secondary structures that are highly preferred sites for BLM cleavage. These conformations appear to be unaffected by nucleoproteins bound to DNA.
对博来霉素诱导纯化的和细胞内猿猴病毒40（SV40）DNA切割的位点进行了检测。纯化DNA中的断裂位点被定位到几个离散的位点，这些位点分布在整个病毒基因组中，但与共同的遗传元件无关。双链断裂发生在第一个单链切口的位置，切割区域不受DNA超螺旋变化的影响。博来霉素在纯化DNA中被切割的相同位点切割细胞内染色体。这些结果表明，SV40 DNA含有DNA二级结构，这些结构是博来霉素切割的高度优先位点。这些构象似乎不受与DNA结合的核蛋白的影响。
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