干燥脐带是用于回顾性诊断宫内肠道病毒感染的潜在材料。
Dried umbilical cord is a potential material for retrospective diagnosis of intrauterine enterovirus infection.
作者信息
Morioka Ichiro, Matsumoto Masaaki, Miwa Akihiro, Yokota Tomoyuki, Matsuo Kiyomi, Koda Tsubasa, Nagasaka Miwako, Shibata Akio, Fujita Kaori, Yamane Masayuki, Yamada Hideto, Enomoto Miki, Chikahira Masatsugu, Iijima Kazumoto
机构信息
Department of Pediatrics, Kobe University Hospital , Kobe , Japan .
出版信息
J Matern Fetal Neonatal Med. 2014 Nov;27(17):1820-2. doi: 10.3109/14767058.2013.879701. Epub 2014 Feb 3.
OBJECTIVE
To determine whether dried umbilical cords (UCs) are useful for retrospective diagnosis of intrauterine enterovirus (EV) infection.
METHODS
Dried UCs in two patients with neonatal EV sepsis and 10 neonates without infectious signs were enrolled. Viral RNA was extracted from their dried UCs, and nested reverse transcription polymerase chain reaction (RT-PCR) was performed.
RESULTS
Infection routes estimated by the clinical course were intrauterine infection in Case 1 and post-natal horizontal infection in Case 2. EV-RNA was detected from dried UC in Case 1, but not in Case 2 and 10 neonates.
CONCLUSIONS
This report showed the potential use of dried UCs for retrospective diagnosis of intrauterine EV infection.
目的
确定干燥脐带是否有助于回顾性诊断宫内肠道病毒（EV）感染。
方法
纳入两名新生儿EV败血症患者及10名无感染体征的新生儿的干燥脐带。从其干燥脐带中提取病毒RNA，并进行巢式逆转录聚合酶链反应（RT-PCR）。
结果
根据临床病程估计，病例1的感染途径为宫内感染，病例2为出生后水平感染。病例1的干燥脐带中检测到EV-RNA，病例2及10名新生儿的干燥脐带中未检测到。
结论
本报告显示干燥脐带在回顾性诊断宫内EV感染方面具有潜在用途。
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