Department of Botany, Patna University, Bihar 800005, India.
Department of Biomedical Sciences, SRCASW, University of Delhi, Vasundhara Enclave, New Delhi 110096, India.
Gene. 2014 Mar 10;537(2):189-96. doi: 10.1016/j.gene.2014.01.007. Epub 2014 Jan 13.
An in-silico analysis of simple sequence repeats (SSRs) in genomes of 32 species of potexviruses was performed wherein a total of 691 SSRs and 33 cSSRs were observed. Though SSRs were present in all the studied genomes their incident frequency ranged from 11 to 30 per genome. Further, 10 potexvirus genomes possessed no cSSRs when extracted at a dMAX of 10 and wherein present, the highest frequency was 3. SSR and cSSR incidence, relative density and relative abundance were non-significantly correlated with genome size and GC content suggesting an ongoing evolutionary and adaptive phase of the virus species. SSRs present primarily ranged from mono- to tri-nucleotide repeat motifs with a greatly skewed distribution across the coding and non-coding regions. Present work is an effort for the undergoing compilation and analysis of incidence, distribution and variation of the viral repeat sequences to understand their evolutionary and functional relevance.
对 32 种马铃薯 Y 病毒科病毒基因组中的简单重复序列(SSR)进行了计算机分析,共观察到 691 个 SSR 和 33 个 cSSR。尽管 SSR 存在于所有研究的基因组中,但它们的发生率范围为每个基因组 11 到 30。此外,当提取的 dMAX 为 10 时,10 个马铃薯 Y 病毒科基因组没有 cSSR,而在存在的情况下,频率最高为 3。SSR 和 cSSR 的发生率、相对密度和相对丰度与基因组大小和 GC 含量没有显著相关性,表明病毒种处于持续的进化和适应阶段。存在的 SSR 主要从单核苷酸到三核苷酸重复基序,在编码区和非编码区的分布存在很大的偏斜。目前的工作是对病毒重复序列的发生、分布和变异进行编目和分析,以了解其进化和功能相关性。