用 [(68)Ge]锗酸作为示踪剂研究硅藻对硅酸的吸收。

Silicic-acid uptake in diatoms studied with [(68)Ge]germanic acid as tracer.

机构信息

Institute of Marine Resources, University of California/San Diego, 92037, La Jolla, California, USA.

出版信息

Planta. 1974 Jan;121(3):205-12. doi: 10.1007/BF00389321.

Abstract

Uptake of silicic acid in the diatoms Navicula pelliculosa and Nitzschia alba was studied, using the natural isotope, (28)Si, or a radioisotope, (31)Si. The rate of uptake of silicic acid was also determined by using [(68)Ge]germanic acid as a tracer of silicic acid. At a given silicicacid concentration in the growth medium, the fractions of [(68)Ge]germanic acid taken up followed closely the fraction of silicic acid taken up in the same time period. When the initial concentration of silicic acid was varied at a constant (trace) concentration of [(68)Ge]-germanic acid, the uptake of (68)Ge followed the fraction of silicic acid removed, and not the absolute amount removed from the medium, at all silicic-acid concentrations. The usefulness of this approach in studying silicic-acid uptake is discussed.

摘要

使用天然同位素（28）Si 或放射性同位素（31）Si 研究了硅藻颗粒和白盘藻对硅酸的吸收。还使用 [(68)Ge] 锗酸作为硅酸示踪剂来确定硅酸的吸收速率。在生长培养基中给定的硅酸浓度下，[(68)Ge] 锗酸的吸收分数与同一时间段内硅酸的吸收分数密切相关。当 [(68)Ge]- 锗酸的初始浓度恒定时（示踪），硅酸的初始浓度变化时，无论在所有硅酸浓度下，(68)Ge 的吸收都遵循硅酸被去除的分数，而不是从中去除的绝对量从培养基中。讨论了这种方法在研究硅酸吸收中的有用性。