Lu Ying-Xia, Gu Qing-Long, Du Jun, Zhao Jing, Liu Chen, Huang Xiao-Lan, Zou Ji-Zhen
Department of Otolaryngology-Head and Neck Surgery, Capital Institute of Pediatrics affiliated children's hospital, Beijing, China.
Department of Otolaryngology-Head and Neck Surgery, Capital Institute of Pediatrics affiliated children's hospital, Beijing, China.
Int J Pediatr Otorhinolaryngol. 2014 Mar;78(3):445-50. doi: 10.1016/j.ijporl.2013.12.010. Epub 2013 Dec 19.
The aim of the study was to investigate upper airway cough syndrome (UACS) in children and to determine alternative methods to explore the relationships among TRPV1, TGF-β2, and UACS.
In 2012, 104 children with adenoid hypertrophy aged 2-13 years who were admitted to the otolaryngology department, Capital Institute of Pediatrics-affiliated children's hospital, were included in this study. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical (IHC) studies for TRPV1 and TGF-β2 were performed to understand the relationship between the two inflammatory factors, and the correlations among the indices and UACS. The research was divided into three stages. In stage 1, 72 children (24 UACS and 48 controls) were enrolled in the study, and ELISAs for TRPV1 and TGF-β2 were performed. In stage 2, 32 children (16 UACS and 16 controls) were enrolled in the study and both ELISA and IHC for TRPV1 and TGF-β2 were performed. In stage 3, 41 children were enrolled in this research who had thick mucus secretions in the posterior nasal apertures in stage 1 and 2 (23 cases with chief complaint (or history) of chronic cough and 18 cases without). The difference between the TRPV1 and TGF-β2 serum values and the clinical factors was determined.
The levels of TRPV1 and TGF-β2 were significantly increased in the UACS cases. OSAHS and thick mucus secretions correlated with a diagnosis of UACS. A history of asthma and thick mucus secretions correlated with elevation of the two inflammatory factors. There was no statistical correlation between ELISA and IHC testing. Among the children with thick mucus secretions, some had a higher possibility of chronic coughing including those who had higher levels of the two indices, larger tonsils and a history of chronic tonsillitis.
The detections of TRPV1 and TGF-β2 from serum and adenoid body specimens are valuable for UACS auxiliary diagnosis. Tonsil hypertrophy and chronic tonsillitis history are independent risk factors of UACS.
本研究旨在调查儿童上气道咳嗽综合征（UACS），并确定探索瞬时受体电位香草酸亚型1（TRPV1）、转化生长因子-β2（TGF-β2）与UACS之间关系的替代方法。
2012年，纳入首都儿科研究所附属儿童医院耳鼻咽喉科收治的104例2至13岁腺样体肥大儿童。对TRPV1和TGF-β2进行酶联免疫吸附测定（ELISA）和免疫组织化学（IHC）研究，以了解这两种炎症因子之间的关系，以及各项指标与UACS之间的相关性。研究分为三个阶段。第一阶段，72例儿童（24例UACS和48例对照）纳入研究，进行TRPV1和TGF-β2的ELISA检测。第二阶段，32例儿童（16例UACS和16例对照）纳入研究，同时进行TRPV1和TGF-β2的ELISA和IHC检测。第三阶段；41例在第一和第二阶段后鼻孔有浓稠黏液分泌物的儿童纳入本研究（23例以慢性咳嗽为主诉（或病史），18例无）。确定TRPV1和TGF-β2血清值与临床因素之间的差异。
UACS病例中TRPV1和TGF-β2水平显著升高。阻塞性睡眠呼吸暂停低通气综合征（OSAHS）和浓稠黏液分泌物与UACS诊断相关。哮喘病史和浓稠黏液分泌物与两种炎症因子升高相关。ELISA和IHC检测之间无统计学相关性。在有浓稠黏液分泌物的儿童中，一些儿童慢性咳嗽的可能性较高，包括那些两种指标水平较高、扁桃体较大且有慢性扁桃体炎病史的儿童。
血清和腺样体标本中TRPV1和TGF-β2的检测对UACS辅助诊断有价值。扁桃体肥大和慢性扁桃体炎病史是UACS的独立危险因素。
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