体外诱导乙肝表面抗原特异性CD8 CD11人抑制性T细胞
In vitro induction of HBsAg-specific CD8 CD11 human suppressor T cells.
作者信息
Barnaba V, Ruberti G, Levrero M, Balsano F
机构信息
Istituto I Clinica Medica, Università La Sapienza, Rome, Italy.
出版信息
Immunology. 1987 Nov;62(3):431-8.
Anti-hepatitis B surface (HBs) antibodies have been induced in vitro by human peripheral blood mononuclear cells (PBMC) from individuals immunized with hepatitis B surface antigen (HBsAg). Anti-HBs antibody production is antigen-specific, T-cell dependent, class II MHC-restricted and requires de novo synthesis. Furthermore, HBsAg-specific CD8 suppressor cells can also be induced in vitro after challenge with high antigen doses. Antigen presenting cells (APC) are required for the induction of suppression. These suppressor cells are antigen-specific since they do not suppress the antibody response to tetanus toxoid. Antigen-specific suppression is inhibited by cytotoxic treatment of CD8 CD11 cells with OKM1 monoclonal antibody (MoAb) and complement, suggesting that these suppressor CD8 cells may represent granular lymphocytes. Addition to cultures of high concentrations of a recombinant human IL-2 dose not affect suppression, ruling out the adsorption of IL-2 by suppressor cells as a possible mechanism for suppression.
来自接种乙肝表面抗原（HBsAg）个体的人外周血单个核细胞（PBMC）已在体外诱导产生抗乙肝表面（HBs）抗体。抗-HBs抗体的产生具有抗原特异性、T细胞依赖性、II类主要组织相容性复合体（MHC）限制性，且需要从头合成。此外，高剂量抗原刺激后，HBsAg特异性CD8抑制细胞也可在体外诱导产生。抑制作用的诱导需要抗原呈递细胞（APC）。这些抑制细胞具有抗原特异性，因为它们不抑制对破伤风类毒素的抗体反应。用OKM1单克隆抗体（MoAb）和补体对CD8 CD11细胞进行细胞毒性处理可抑制抗原特异性抑制作用，这表明这些抑制性CD8细胞可能代表颗粒淋巴细胞。向培养物中添加高浓度的重组人白细胞介素-2（IL-2）剂量并不影响抑制作用，排除了抑制细胞吸附IL-2作为一种可能的抑制机制。
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