一种通过 HRP 模拟 DNA zyme-肽偶联物监测 caspase 3 活性的新比色策略。

A new colorimetric strategy for monitoring caspase 3 activity by HRP-mimicking DNAzyme-peptide conjugates.

机构信息

Department of Chemistry, Beijing Key Laboratory for Microanalytical Methods and Instrumentation, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.

出版信息

Analyst. 2014 Mar 7;139(5):1178-83. doi: 10.1039/c3an02028b. Epub 2014 Jan 21.

DOI:10.1039/c3an02028b

PMID:24445423

Abstract

A new method for caspase 3 activity assay has been developed based on HRP-mimicking DNAzyme-peptide conjugates. The mechanism of detection was based on the specific cleavage of DEVD-peptides by active caspase 3 for recognition and the catalytic properties of HRP-mimicking DNAzymes for signal amplification. Under optimal conditions, the detection limit of caspase 3 was 0.89 nM. The proposed method was also successfully applied for the detection of caspase 3 in apoptosis cell lysates.

摘要

一种新的基于 HRP 模拟 DNA 酶-肽偶联物的 caspase 3 活性测定方法已经被开发出来。检测的机制基于活性 caspase 3 对 DEVD-肽的特异性切割进行识别和 HRP 模拟 DNA 酶的催化特性进行信号放大。在最佳条件下，caspase 3 的检测限为 0.89 nM。该方法还成功地应用于凋亡细胞裂解液中 caspase 3 的检测。