一种用于龋齿替代疗法的新型 gcrR 缺陷变形链球菌突变体。
A new gcrR-deficient Streptococcus mutans mutant for replacement therapy of dental caries.
作者信息
Pan Wenting, Mao Tiantian, Xu Qing-an, Shao Jin, Liu Chang, Fan Mingwen
机构信息
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-Most) & Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237 Luoyu Road, Wuhan 430079, China.
Department of Stomatology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430060, China.
出版信息
ScientificWorldJournal. 2013 Dec 18;2013:460202. doi: 10.1155/2013/460202. eCollection 2013.
BACKGROUND
gcrR gene acts as a negative regulator related to sucrose-dependent adherence in S. mutans. It is constructive to test the potential capacity of mutans with gcrR gene deficient in bacteria replacement therapy.
METHODS
In this study, we constructed the mutant by homologous recombination. The morphological characteristics of biofilms were analyzed by confocal laser scanning microscopy. S. mutans UA159 and the mutant MS-gcrR-def were inoculated, respectively, or together for competitive testing in vitro and in rat model.
RESULTS
Adhesion assay showed that the adhesion ability of the mutant increased relative to the wild type, especially in the early stage. MS-gcrR-def out-competed S. mutans UA159 in vitro biofilm, and correspondingly coinfection displayed significantly fewer caries in vivo. The former possessed both a lower level of acid production and a stronger colonization potential than S. mutans UA159.
CONCLUSION
These findings demonstrate that MS-gcrR-def appears to be a good candidate for replacement therapy.
背景
gcrR基因作为变形链球菌中与蔗糖依赖性黏附相关的负调控因子。测试缺乏gcrR基因的变形链球菌在细菌替代疗法中的潜在能力具有重要意义。
方法
在本研究中，我们通过同源重组构建了突变体。利用共聚焦激光扫描显微镜分析生物膜的形态特征。分别接种变形链球菌UA159和突变体MS-gcrR-def，或一起接种进行体外和大鼠模型中的竞争测试。
结果
黏附试验表明，突变体的黏附能力相对于野生型有所增加，尤其是在早期。MS-gcrR-def在体外生物膜中比变形链球菌UA159更具竞争力，相应地，共感染在体内显示出明显更少的龋齿。前者比变形链球菌UA159具有更低的产酸水平和更强的定植潜力。
结论
这些发现表明MS-gcrR-def似乎是替代疗法的良好候选者。
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