RhoC 通过 Rho 相关丝氨酸/苏氨酸蛋白激酶 1 信号通路介导 CaSki 细胞的侵袭和迁移。
RhoC mediates invasion and migration of CaSki cells through the Rho-associated serine-threonine protein kinase 1 signaling pathway.
机构信息
*Department of Gynecology and Obstetrics, and †Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Cardiovascular Molecular Biology and Regulatory Peptide, Ministry of Health, Beijing, China.
出版信息
Int J Gynecol Cancer. 2014 Feb;24(2):184-91. doi: 10.1097/IGC.0000000000000053.
OBJECTIVE
The small GTPase RhoC in human cancers is up-regulated and correlated with tumor metastasis. However, the role of Rho/Rho-associated serine-threonine protein kinase 1 (ROCK1) signaling pathway in human cervical cancer is still unclear. In this study, we examine the effects of RhoC and its major downstream target, ROCK1, on the invasion and migration of CaSki cells to investigate the role of RhoC/ROCK1 signaling pathway in the progression of cervical squamous cell carcinoma.
METHODS
RhoC and ROCK1 protein expression in CaSki cells was detected by Western blotting. Scratch and transwell assays were carried out to assess the effects of RhoC on invasion and migration of CaSki cells. Cell viability was assayed by MTT test after adding the ROCK1 inhibitor Y-27632 to CaSki cells.
RESULTS
Overexpression of RhoC protein in CaSki cells significantly increases ROCK1 expression and promotes cell invasion and migration compared with the control group (P < 0.05). However, in the inhibition of ROCK1 with Y-27632 in CaSki cells when RhoC was overexpressed, the rate of invasiveness and migration was reduced remarkably (P < 0.05), dropping to comparable levels as the control.
CONCLUSIONS
This study suggested that the activation of RhoC/ROCK1 signaling pathways is likely involved in the progression of cervical squamous cell carcinoma.
目的
人类癌症中的小 GTPase RhoC 上调，并与肿瘤转移相关。然而，Rho/Rho 相关丝氨酸-苏氨酸蛋白激酶 1（ROCK1）信号通路在人宫颈癌中的作用仍不清楚。在本研究中，我们研究了 RhoC 及其主要下游靶点 ROCK1 对 CaSki 细胞侵袭和迁移的影响，以探讨 RhoC/ROCK1 信号通路在宫颈鳞状细胞癌进展中的作用。
方法
通过 Western blot 检测 CaSki 细胞中 RhoC 和 ROCK1 蛋白的表达。划痕和 Transwell 实验评估 RhoC 对 CaSki 细胞侵袭和迁移的影响。在加入 ROCK1 抑制剂 Y-27632 后，通过 MTT 试验检测细胞活力。
结果
与对照组相比，RhoC 蛋白在 CaSki 细胞中的过表达显著增加了 ROCK1 的表达，并促进了细胞侵袭和迁移（P<0.05）。然而，当 RhoC 过表达时，用 Y-27632 抑制 ROCK1，侵袭和迁移的速率显著降低（P<0.05），降至与对照组相当的水平。
结论
本研究表明 RhoC/ROCK1 信号通路的激活可能参与了宫颈鳞状细胞癌的进展。
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