人SOX18蛋白新型显性负性突变体的构建与功能分析
Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein.
作者信息
Milivojevic M, Petrovic I, Kovacevic-Grujicic N, Popovic J, Mojsin M, Stevanovic M
机构信息
Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, 11010, Serbia.
出版信息
Biochemistry (Mosc). 2013 Nov;78(11):1287-92. doi: 10.1134/S0006297913110096.
SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
SOX18转录因子在一系列生物学过程中发挥着重要作用，如血管生成、毛囊发育、淋巴管生成、动脉粥样硬化和血管生成。在本文中，我们展示了一种新型SOX18显性负性突变体（SOX18DN）的产生，该突变体编码缺少反式激活结构域的截短SOX18蛋白。我们表明，野生型SOX18（SOX18wt）和截短的人SOX18蛋白在体外都能够结合它们的共有序列。功能分析证实，SOX18wt具有强大的反式激活特性，而SOX18DN表现出显性负性效应。我们相信，这些SOX18wt和SOX18DN表达构建体可成功用于进一步表征该蛋白的功能。
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