Castro M R
Instituto Municipal de Investigacion Medica, Barcelona, Spain.
Allergol Immunopathol (Madr). 1987 Jul-Aug;15(4):225-8.
The thymus-independent antigen Dextran B512 was used to induce tolerance with immunogenic doses. We show the difficulty to obtain a steady state by administering weekly antigen doses. Three weekly doses were required to achieve tolerance or "homeostatic equilibrium". Three groups of mice (C57BL6 X CBA) F1 were stimulated with: a) 0.5 microgram Dextran one weekly dose for ten weeks. b) 2 micrograms Dex. one weekly dose, for twenty-two weeks, and c) 10 micrograms Dex. three times a week, administered continuously for eleven weeks. The number of anti-Dex. antibody secreting cells was determined by Cunningham and Szenberg PFC technique. Weekly continuous doses of 0.5 and 2 micrograms of antigen showed frequent fluctuations in IgM and unresponsiveness was not achieved. Nonetheless, a three times weekly dose of 10 micrograms Dex. showed IgM response with oscillations and little anti-Dex. IgG response was obtained. After eleven weeks of continuous stimulation the steady state was succeeded. This antigen triggered high levels of IgM bearing B cells in mice, and more difficult tolerance induction was known in this isotype, but also the stimulation model was an important fact in achievement of steady state.
使用非胸腺依赖性抗原葡聚糖B512以免疫原性剂量诱导耐受。我们展示了通过每周给予抗原剂量来获得稳态的困难。需要三周的剂量才能实现耐受或“稳态平衡”。三组（C57BL6×CBA）F1小鼠分别用以下方式刺激：a）每周一次给予0.5微克葡聚糖，共十周。b）每周一次给予2微克葡聚糖，共二十二周，以及c）每周三次给予10微克葡聚糖，连续给药十一周。通过坎宁安和森伯格的PFC技术测定抗葡聚糖抗体分泌细胞的数量。每周连续给予0.5微克和2微克抗原显示IgM频繁波动，未实现无反应性。尽管如此，每周三次给予10微克葡聚糖显示出IgM有振荡反应，且几乎没有获得抗葡聚糖IgG反应。连续刺激十一周后成功达到稳态。这种抗原在小鼠中触发了高水平的携带IgM的B细胞，并且已知在这种同种型中诱导耐受更困难，但刺激模型也是实现稳态的一个重要因素。
