Departamento de Química Analítica, Nutrición y Bromatología e Instituto Universitario de Materiales, Facultad de Ciencias, Universidad de Alicante, P.O. Box 99, E-03080 Alicante, Spain.
Laboratory of Aquatic Chemistry, School of Environmental Engineering, Technical University of Crete, Polytechneioupolis, GR-73100 Chania, Greece.
J Chromatogr A. 2014 Feb 21;1330:1-5. doi: 10.1016/j.chroma.2014.01.003. Epub 2014 Jan 13.
Vortex-assisted liquid-liquid microextraction (VALLME) coupled with high-performance liquid chromatography (HPLC) is proposed here for the rapid determination of octanol-water partitioning coefficients (Kow). VALLME uses vortex agitation, a mild emulsification procedure, to disperse microvolumes of octanol in the aqueous phase thus increasing the interfacial contact area and ensuring faster partitioning rates. With VALLME, 2min were enough to achieve equilibrium conditions between the octanolic and aqueous phases. Upon equilibration, separation was achieved using centrifugation and the octanolic microdrop was collected and analyzed in a HPLC system. Six model compounds with logKow values ranging between ∼0.5 and 3.5 were used during the present investigations. The proposed method produced logKow values that were consistent with previously published values and the recorded uncertainty was well within the acceptable log unit range. Overall, the key features of the proposed Kow determination procedure comprised speed, reliability, simplicity, low cost and minimal solvent consumption.
涡旋辅助液 - 液微萃取（VALLME）与高效液相色谱（HPLC）相结合，用于快速测定辛醇 - 水分配系数（Kow）。VALLME 使用涡旋搅拌，一种温和的乳化程序，将微体积的辛醇分散在水相中，从而增加界面接触面积并确保更快的分配速率。使用 VALLME，2 分钟即可在辛醇相和水相之间达到平衡条件。达到平衡后，通过离心实现分离，并在 HPLC 系统中分析辛醇微滴。在目前的研究中，使用了六种具有 logKow 值在 ∼0.5 到 3.5 之间的模型化合物。所提出的方法产生的 logKow 值与先前发表的值一致，记录的不确定性在可接受的对数单位范围内。总的来说，所提出的 Kow 测定程序的主要特点是速度、可靠性、简单性、低成本和最小的溶剂消耗。
Zotero 插件