Willenbrink J, Kremer B P
Botanisches Institut der Universität zu Köln, Gyrhofstraße 15, D-5000, Köln 41, Federal Republic of Germany.
Planta. 1973 Jun;113(2):173-8. doi: 10.1007/BF00388201.
Tips of fronds of Fucus serratus L. were exposed to H(14)CO3 in the light for periods of 10, 30, 60, and 180s, fixed in petrol ether at-70° C, and subsequently lyophilized. Pheoplasts (=chloroplasts) were isolated using the nonaqueous technique of Thalacker et al. (1959). After extraction and chromatography percentage (14)C activity and distribution of individual photoassimilates between pheoplasts and other compartments of assimilating cells were analyzed. Eighty percent of [(14)C]-phosphate esters were found within the pheoplasts after 10s (14)C-assimilation, whereas only 25% were found there after 30s. After 10s [(14)C] mannitol is almost totally localized within the plastids, but after 180s the major part has been localized outside the pheoplasts. On the basis of these data the pheoplasts are regarded to be the only sites of primary mannitol biosynthesis during photosynthesis in Fucus.
将锯齿墨角藻（Fucus serratus L.）的叶状体尖端在光照下暴露于H(14)CO3中10、30、60和180秒，于-70°C下用石油醚固定，随后进行冻干。使用Thalacker等人（1959年）的非水技术分离质体（=叶绿体）。在提取和色谱分析后，分析了质体与同化细胞其他区室之间单个光合产物的(14)C活性百分比和分布。在14C同化10秒后，80%的[(14)C]-磷酸酯存在于质体中，而在30秒后只有25%存在于质体中。在10秒后，[(14)C]甘露醇几乎完全定位于质体内，但在180秒后，大部分已定位于质体之外。基于这些数据，质体被认为是锯齿墨角藻光合作用期间初级甘露醇生物合成的唯一部位。
