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采用气相色谱-质谱联用法鉴定落地生根中赤霉素 A20、脱落酸和法呢基焦磷酸。

Identification of gibberellin A20, abscisic acid, and phaseic acid from flowering Bryophyllum daigremontianum by combined gas chromatography-mass spectrometry.

机构信息

School of Chemistry, University of Bristol, BS8 1TS., Bristol, U.K..

出版信息

Planta. 1973 Dec;111(4):347-52. doi: 10.1007/BF00385554.

DOI:10.1007/BF00385554
PMID:24469701
Abstract

The presence of abscisic and phaseic acid in a purified acidic extract from flowering plants of the long-short-day plant Bryophyllum daigremontianum [(R. Hamet and Perr.) Berg.] was conclusively established by combined gas chromatography-mass spectrometry (GC-MS) of their methyl esters. Gibberellin A20 (GA20) was identified by GC-MS of the methyl ester and the trimethylsilyl ether of the methyl ester. The following levels of the 3 compounds per kg fresh weight were estimated: Abscisic acid, 5.5 μg; phaseic acid, 9.4μg; gibberellin A20, 0.8 μg. When GA20 and four other GAs were applied to Bryophyllum under shortday conditions, the order of effectiveness for induction of flower formation was: GA2>GA1>GA5=GA7>GA20. The low biological activity of the native GA20 is discussed.

摘要

用气相色谱-质谱(GC-MS)对其甲酯进行分析,明确证实了短日照植物落地生根(Bryophyllum daigremontianum [(R. Hamet and Perr.) Berg.])花中纯化酸性提取物中存在脱落酸和法呢基酸。用甲酯和甲酯的三甲基硅醚的 GC-MS 鉴定出赤霉素 A20(GA20)。每公斤鲜重估计有以下 3 种化合物的含量:脱落酸 5.5μg;法呢基酸 9.4μg;赤霉素 A200.8μg。当 GA20 和其他四种 GA 在短日照条件下施加于落地生根时,诱导花形成的效果顺序为:GA2>GA1>GA5=GA7>GA20。对天然 GA20 的低生物活性进行了讨论。

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Identification of gibberellin A20, abscisic acid, and phaseic acid from flowering Bryophyllum daigremontianum by combined gas chromatography-mass spectrometry.采用气相色谱-质谱联用法鉴定落地生根中赤霉素 A20、脱落酸和法呢基焦磷酸。
Planta. 1973 Dec;111(4):347-52. doi: 10.1007/BF00385554.
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3
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本文引用的文献

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Planta. 1969 Jun;86(2):124-33. doi: 10.1007/BF00379820.
2
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Planta. 1969 Dec;84(4):339-47. doi: 10.1007/BF00396425.
3
The occurrence of abscisic acid in inhibitors B1 and C from immature fruit of Ceratonia siliqua L. (carob) and in commercial carob syrup.
长日照和短日照条件下落地生根植物中[(3)H]赤霉素 A20 的代谢。
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4
Identification of gibberellins in the rice plant and quantitative changes of gibberellin A19 throughout its life cycle.鉴定水稻中的赤霉素和赤霉素 A19 在其整个生命周期中的定量变化。
Planta. 1979 Jan;146(2):185-91. doi: 10.1007/BF00388230.
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Planta. 1981 Nov;153(3):238-45. doi: 10.1007/BF00383893.
从刺槐豆(角豆)未成熟果实的抑制剂 B1 和 C 以及商业角豆糖浆中发现脱落酸。
Planta. 1970 Mar;92(1):41-9. doi: 10.1007/BF00385561.
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Plant Physiol. 1971 Jun;47(6):821-7. doi: 10.1104/pp.47.6.821.
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