Karabey Tuçe, Sert Osman
Faculty of Science Department of Biology, Hacettepe University, Beytepe Campus, 06532, Ankara, Turkey.
Int J Legal Med. 2018 Jul;132(4):1185-1196. doi: 10.1007/s00414-014-0968-2. Epub 2014 Jan 30.
Forensic entomology is a study that insect evidence is used for the determination of postmortem interval (PMImin). While determining PMImin, the pupal period is given as the duration between the beginning of the pupal period and the stage of adult emergence. The pupal period constitutes approximately 50 % of the immature development, and with detailed examination it would be possible to present a much more accurate PMImin. In this study, the pupal period of Lucilia sericata (Meigen, 1826), which is one of the species used in PMImin estimation in forensic entomology, was investigated. Studies about the pupal period of development are considerably limited in the forensic entomology world. Stages and duration of the pupal period of Lucilia sericata were studied at temperatures of 20 °C, 25 °C, and 30 °C. For all temperatures, pupae specimens were dissected and photographed hourly. According to findings based on all three temperatures, it was determined that as the temperature increases, the duration of development shortens. Also, in addition to 11 pre-determined development stages, nine new development stages were identified.
法医昆虫学是一项利用昆虫证据来确定死后间隔时间（最短死后间隔时间，PMImin）的研究。在确定最短死后间隔时间时，蛹期被定义为从蛹期开始到成虫羽化阶段的持续时间。蛹期约占幼虫发育阶段的50%，通过详细检查有可能得出更准确的最短死后间隔时间。在本研究中，对丝光绿蝇（Lucilia sericata，梅根，1826年）的蛹期进行了调查，丝光绿蝇是法医昆虫学中用于估计最短死后间隔时间的物种之一。在法医昆虫学界，关于蛹期发育的研究相当有限。在20℃、25℃和30℃的温度下研究了丝光绿蝇蛹期的阶段和持续时间。对于所有温度，每小时对蛹标本进行解剖和拍照。根据基于所有这三个温度的研究结果，确定随着温度升高，发育持续时间缩短。此外，除了11个预先确定的发育阶段外，还识别出了9个新的发育阶段。
