从 Xenorhabdus bovienii 中分离的一种蛋白酶抑制剂蛋白对豌豆蚜（Acyrthosiphon pisum）生理的影响。
Effects of a protease inhibitor protein from Xenorhabdus bovienii on physiology of pea aphid (Acyrthosiphon pisum).
机构信息
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Beijing 100081, PR China; Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, PR China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Beijing 100081, PR China.
出版信息
Pestic Biochem Physiol. 2014 Jan;108:86-91. doi: 10.1016/j.pestbp.2013.12.010. Epub 2014 Jan 9.
The effects of a protease inhibitor protein from Xenorhabdus bovienii strain xbi1 (Xbpi-1) on biochemistry and physiology of pea aphid (Acyrthosiphon pisum) were studied, and the effects of Xbpi-1 on the activities of aphid aminopeptidase and the concentrations of carbohydrates, free amino acids and lipids in A. pisum were determined. The results showed that the activity of aminopeptidase was reduced 18.30% by Xbpi-1 in vitro assays. In addition, the total sugar, glycogen, and lipid concentrations per aphid fed on an artificial diet with Xbpi-1 were reduced by 39.41%, 24.61% and 26.25%, respectively, compared to the controls. The total free amino acids in the aphids on the diet with Xbpi-1 was significantly decreased by 20.78% compared to the control. The mortalities of A. pisum were significantly higher when the concentrations of Xbpi-1 were 400 and 800 μg/ml in the artificial diet. The results from this study indicated that Xbpi-1 had adverse effects on the physiology and biochemistry of A. pisum.
研究了从 Xenorhabdus bovienii 菌株 xbi1（Xbpi-1）中提取的蛋白酶抑制剂蛋白对豌豆蚜（Acyrthosiphon pisum）的生物化学和生理学的影响，并测定了 Xbpi-1 对蚜虫氨肽酶活性以及豌豆蚜体内碳水化合物、游离氨基酸和脂类浓度的影响。结果表明，Xbpi-1 在体外试验中可使氨肽酶活性降低 18.30%。此外，与对照组相比，喂食含 Xbpi-1 的人工饲料的蚜虫的总糖、糖原和脂类浓度分别降低了 39.41%、24.61%和 26.25%。含 Xbpi-1 的饲料中的蚜虫总游离氨基酸含量比对照组显著降低了 20.78%。当人工饲料中 Xbpi-1 的浓度分别为 400 和 800μg/ml 时，豌豆蚜的死亡率显著升高。本研究结果表明，Xbpi-1 对 A. pisum 的生理和生物化学有不良影响。
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